甘蓝型油菜‘南盐油1号’不同外植体再生体系的比较研究

The comparative study on different explant regeneration systems in canola‘Nanyanyou 1'(Brassica napus L.)

以甘蓝型油菜‘南盐油1号’下胚轴和具柄子叶为材料,通过植物组织培养方法,探讨不同预培养和分化培养的交叉组合对油菜植株再生的影响。结果表明:不同浓度的2,4-D、6-BA和AgNO3组合对外植体的分化有不同程度的影响,但分化出的芽苗均能生根,并生长成为植株。下胚轴在预培养(MS+0.5 mg.L-12,4-D+1.0 mg.L-16-BA)和分化培养(MS+3.0 mg.L-16-BA+0.1 mg.L-1NAA+5.0 mg.L-1AgNO3)条件下的分化率最高,达到95.23%;具柄子叶在预培养(MS+0.5 mg.L-12,4-D+1.0 mg.L-16-BA)和分化培养(MS+4.5 mg.L-16-BA+0.1 mg.L-1NAA+5.0 mg.L-1AgNO3)下的分化率最高,达到89.52%。上述结果表明,下胚轴很可能更适合作为‘南盐油1号’油菜在组织培养研究中的外植体。

The plant tissue culture was tried in the experiment,in which hypocotyl and the cotyledon with petiole of＇Nanyanyou 1＇（Brassica napus L.）were used as materials.The effects of cross combination of the different pre-culture and differentiation culture on the canola plant regeneration were studied.The results showed that different concentrations of 2,4-D,6-BA and AgNO3 had varing degree of influences on explant differentiation,however,differentiated buds were all able to take root and grow into plant.The differentiation rate of hypocotyl achieved the peak value（95.23%）in the pre-culture（MS＋0.5 mg·L-12,4-D＋1.0 mg·L-1 6-BA）and differentiation（MS＋3.0 mg·L-1 6-BA＋0.1 mg·L-1 NAA＋5.0 mg·L-1 AgNO3）;the cotyledon with petiole achieved the peak value（89.52%）in pre-culture（MS＋0.5 mg·L-1 2,4-D＋1.0 mg·L-1 6-BA）and differentiation（MS＋4.5 mg·L-1 6-BA＋0.1 mg·L-1 NAA＋5.0 mg·L-1 AgNO3）.To sum up,compared with the cotyledon with petiole,hypocotyl might be more suitable as the explant in tissue culture research of＇Nanyanyou 1＇.