提取方式对山豆根“质量-毒性”综合评价模式的影响

The Effeet of Extracting Methods on "Quality Toxicity" Comprehensive Evaluation Model of Rhizome et Rhizome Sophorae Tonkinensis

目的对不同提取方式制备的山豆根样品中毒性物质基础生物碱类物质的质量研究,并结合前期的急性毒性研究,探讨提取方式对山豆根"质量-毒性"综合评价模式的影响。方法用高效液相色谱法测定山豆根水提组分、醇提组分中苦参碱和氧化苦参碱的含量,并进行方法学考察。结果对山豆根不同提取方式制备的样品中苦参碱和氧化苦参碱的含量测定,发现苦参碱的含量大小为:水提组分>醇提组分,氧化苦参碱的含量大小为:醇提组分>水提组分。结论山豆根不同提取方式制备的样品中中苦参碱、氧化苦参碱的含量大小与急性毒性间均有一定的关系,但不完全一致。说明提取方式对北豆根"质量-毒性"综合评价模式有一定的影响,因此通过"质量-毒性"综合评价模式确定有毒中药的提取方式,更全面客观合理。

Objective Explore the effeet of extracting methods on ＂quality-toxicity＂ comprehensive evaluation model by comparing the content of alkaloid and acute toxicity from Radix et Rhizome Sophorae Tonkinensis extracted by different methods, the quality control methodology of alkaloid substance from Radix er Rhizome Sophorae Tonldnensis was studied. Methods HPLC was used to determine the content ofmatrine and oxymatrine in water extract and alcohol extract ofRad/x et Rhizome Sophorae Tonldnensis. Results In the determination ofmatrine and oxymatrine in different components of Radix et Rhizome Sophorae Tonkinensis, it was found that the size order of the content of matrine is water extract〉alcohol extract, and the content of oxymatrine is alcohol extract〉water extract. Conclusion There is a certain relationship between the content of matrine and oxymatrine and acute toxicity in different components of Rada＇x et Rhizome Sophorae Tonldnensis, but not completely consistent. There was certain effeet of extractingmethods on ＂quality toxicity＂ comprehensive evaluation model. It was more comprehensive, objective and reasonable to determine extraction methods of toxic Chinese medicine through the comprehensive evaluation model.