不同低氧暴露对人红细胞硫氧还蛋白过氧化物酶PeroxiredoxinⅡ的影响

Effect of Different Hypoxic Environment Exposure on PeroxiredoxinⅡ

目的:观察模拟高住组和间歇性低氧组经过四周低氧暴露后,人红细胞硫氧还蛋白过氧化物酶Peroxiredoxin2的变化。方法:男性大学生20名随机分成对照组、模拟高住组和间歇性低氧组,三组从事相同的身体活动、学习和生活作息,模拟高住组每天8hr低氧环境睡眠,间歇性低氧组每天安静状态吸低氧(浓度14%-10%)50-60min,连续4周。三组均在实验开始前、结束后按Bruce方案进行力竭运动,并在安静和运动后取血测试Prx2、MDA。在低氧训练实验开始前一天、实验的最后一天取血测定红细胞数目、血红蛋白及红细胞压积。结果:(1)一次性力竭运动后,Prx2的变化不大,而经过四周低氧暴露后,在完成力竭运动前,模拟高住组Prx2有升高趋势,在间歇性低氧组则达到显著性;力竭运动后,Prx2的升高在低氧组都达到了显著性;(2)力竭运动后,三组MDA明显增多,低氧暴露前后低氧组无明显变化;(3)低氧组都未观察到红细胞计数和压积、血红蛋白浓度的明显变化。结论:长时间低氧暴露可诱导Prx2的生成增多。

Objective The paper investigated the effects of different hypoxia exposure on Peroxiredoxin Ⅱ. Methods Twenty healthy male students were randomly assigned to three group with following protocols： control group（ C）, intermittent hypoxia exposure（IH） and mimic lived high and trained low group （MH）. The IH group intermittently inhaled different hypoxic gas mixtures （02 were 14 %, 12%, 10%, respectively）, 50 -60 rain daily for 4 weeks. M H group was exposed to hypoxic environment for 8 hours each night. Three groups completed Bruce protocol exercise on the treadmill before and after hypoxic exposure. Results 1 ） Before hypoxic exposure, there were no significantly changes of Prx2 after exhaustive exercise. After 4 weeks of hypoxic exposure, Prx2 in MH and IH group were significantly increased. 2） MDA in three groups were significantly change after exhaustive exercise. There were no change between before and after hypoxic exposure. 3 ）Changes of RBC ,HCT and Hb were not investigated between three groups after hypoxic exposure. Conclusion 4 weeks hypoxic exposure is effective on impro- ving Prx2.