星点设计-效应面优化法优选连钱草中总黄酮超声提取工艺

Optimization on Extraction of Total Flavonoids from Glechoma longituba with Ultrasonic Wave by Central Composite Design-Response Surface Method

目的星点设计-效应面优化法优选连钱草中总黄酮的提取工艺。方法以甲醇浓度、超声时间和溶剂用量为考察因素,以连钱草总黄酮的含量为评价指标,借助紫外分光光度法,运用效应面法优化超声波提取连钱草总黄酮的工艺条件。结果连钱草的最佳提取条件为甲醇浓度55%、超声90min及8倍量溶剂。结论星点设计-效应面优化法可用于连钱草总黄酮提取工艺的优化,模型预测性良好,提取条件稳定,实验设计方法有较高的可靠性。

OBJECTIVE To study the optimum condition of extraction for the total flavonoids in Glechoma longituba by central composite design-response surface method.METHODS Independent variables were methanol concentration,ultrasonic time and solvent,while dependent variables were the content of total flavonoids.Central composite design-response surface method（CCD-RSM） were used to optimize the extracting process.RESULTS The content of extraction conditions was 55% methanol,ultrasound 90 min and 8 times the amount of solvent.CONCLUSION CCD-RSM can be used to optimize extraction of total flavonoids of Glechoma longituba,and the optimum mathematic model is highly predictive and the experimental design methods have higher reliability.