摘要
海绵相关微生物是药物开发的重要资源.本文利用6种培养基对汕头南澳普遍存在的海绵Halichondria sp.的共附生放线菌进行分离和培养,共得到36株放线菌.基于16S rRNA基因序列的系统发育分析表明,这些放线菌分别属于Streptomyces、Nocardiopsis和Micromonospora属.抗菌活性筛选结果显示,分离菌株分别对Staphylococcus aureus、Bacillus subtilis和Escherichia coli等的生长有抑制作用.同时分子方法筛选显示这些放线菌具有潜在产生聚酮类、角蒽环类和非核糖体多肽类的能力.本研究结果为海绵相关微生物在药物开发方面积累了基础数据.
In this study, actinobacteria isolated fi＇om the marine sponge Ha!ichondria sp. collected in Shantou Nan＇ao of the South China Sea are described. A total of 36 actinobacteria were isolated by 6 different media. The 16S rRNA genes of 36 isolates were cloned and the sequence results showed that the isolates belong to the genera Streptomyces, Nocardiopsis and Micromonospora. All the isolates were screened for antimicrobial actives and genes encoding polyketide synthases（PKS）, angucycline and nonribosomal peptide synthetases（NRPS）. The results show that almost all isolates have PKS, angucycline, NRPS genes and antimicrobial activties in indicators of Staphylococcus aureus, Bacillus subtilis and Escherichia coll.
出处
《汕头大学学报（自然科学版）》
2012年第2期30-34,共5页
Journal of Shantou University：Natural Science Edition
基金
广州市属高校科研项目(10A161)
广州医学院博士启动基金(L95109)
关键词
海绵
放线菌
抗菌活性
sponge
actinobacteria
diversity
