摘要
目的:建立从管花肉苁蓉中快速制备松果菊苷对照品的工艺。方法:运用快速制备液相色谱对管花肉苁蓉浸膏进行分离纯化,得到高纯度的松果菊苷。结果:分离得到的化合物经UV、IR、MS和NMR鉴定为松果菊苷,HPLC测定其纯度为98.38%。结论:本文方法简便、快速,所得产物纯度高,可用于松果菊苷对照品的制备。
This study was aimed to set up an efficient and fast method to separate echinacoside reference material from Cistanche tubulosa （Schrenk） Wight using flash chromatography. Cistanche tubulosa （Schrenk） Wight extract was iso- lated with flash chromatography. And the prepared compound was identified as echinacoside through UV, IR, MS and NMR, with its HPLC purity of 98.38%. The method is simple, fast and efficient, and can be applied to the preparation of echinacoside reference material.
出处
《世界科学技术-中医药现代化》
北大核心
2012年第2期1451-1454,共4页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
科学技术部国际合作科研基金项目(2009DFA31230):基于澳洲TGA药材注册的南药质量评价体系研究
负责人:杨得坡
广东省科技厅省产学研项目(2010B090400533):中药化学对照品制备关键技术及商业化运营平台的构建
负责人:徐新军
关键词
快速制备液相色谱
管花肉苁蓉
松果菊苷
Flash chromatography, Cistanche tubulosa （Schrenk） Wight, echinacoside
