诱骗受体3在口腔鳞癌中的表达及其临床意义

Expression and clinical significance of DcR3 in human oral squamous cell carcinoma

目的:检测口腔鳞癌中诱骗受体3(DcR3)mRNA及其蛋白的表达,探讨DcR3在口腔鳞癌发生、发展中的作用及其临床意义。方法:应用原位杂交及免疫组化检测57例口腔鳞癌及相应癌旁正常组织中DcR3 mRNA及其蛋白的表达情况。结果:口腔鳞癌中DcR3 mRNA及其蛋白阳性表达率高于癌旁正常组织(P<0.05);DcR3 mRNA及其蛋白的表达与分化程度、TNM分期、淋巴结转移有关(P<0.05),与性别、年龄、肿瘤大小无关;原位杂交和免疫组化检测结果具有一致性(P<0.05)。结论:DcR3在口腔鳞癌的发生、发展过程中发挥重要作用,检测DcR3可能有助于判断口腔鳞癌患者的预后。

Objective To determine the expression of DcR3 mRNA and protein in oral squamous cell carcinoma（OSCC）,explore the role of DcR3 in carcinogenesis and progression,invasion and its clinical significance of OSCC.Method The expression of DcR3 mRNA and protein were detected by in situ hybridization and immunohistochemistry in 57 cases of OSCC and 57 cases of normal tissues adjacent tumor as controls.Results The positive expression rates of DcR3 mRNA and protein in OSCC were significantly higher than normal tissues adjacent tumor（P0.05）.The DcR3 mRNA and protein in OSCC were correlated with the degree of differentiation,clinical TNM stage and lymph node metastasis（P0.05）,but not related to sex,age and size of tumor.Concordance results were found in detecting the expression of DcR3 in OSCC by immunohistochemistry and by in situ hybridization（P0.05）.Conclusion The expression of DcR3 in OSCC may play an important role in tumor pathogenesis and progression.DcR3 may be considered as an indicator in predicting prognosis of OSCC.