白藜芦醇预处理小鼠应激性损伤后心功能动态观察

Dynamic observation of cardiac function in mice after resveratrol preconditioning on stress-induced injury

目的观察白藜芦醇预处理小鼠应激性损伤后心功能的动态变化。方法正常成年雄性昆明小鼠60只分为对照组、创伤组、创伤加白藜芦醇(10μmol/L)组,每组20只。Noble-Collip创伤仪建立非致死性创伤小鼠模型,应用高分辨小动物超声成像系统分别于创伤前(0 h)及创伤后5个时间点(12 h、24 h、36 h、48 h、60 h)进行超声心动图检查。结果 (1)对照组各时间点小鼠心功能比较,差异无统计学意义(P>0.05)。(2)与创伤前比较,创伤组小鼠回心血量显著减少;左心室舒张末期容积在创伤后12 h达最低点,较创伤前下降了46.1%(P<0.05);创伤刺激下小鼠心脏功能出现明显的代偿性改变,创伤后12 h心率达到高峰,增加幅度达32.9%(P<0.05);心排血量在各时间点基本处于同一水平,射血分数和短轴缩短率较创伤前分别降低了16%和20%(P<0.05);E/A比值和E峰减速时间较创伤前减少了36.8%和29.1%(P<0.05)。(3)创伤加白藜芦醇组小鼠回心血量亦明显减少(P<0.05),但较创伤组要轻;创伤后12 h,左心室舒张末期容积较创伤前下降了30.8%(P<0.05);左心室射血分数和短轴缩短率较创伤前分别降低了8.4%和17.7%(P<0.05);E/A比值和E峰减速时间较创伤前减少了24.6%和24.1%(P<0.05)。结论白藜芦醇预处理可以显著改善应激性损伤后小鼠心功能,增强小鼠心脏代偿能力。

Objectives To observe the dynamic changes in cardiac functions after resveratrol preconditioning in mice with stress-induced injury.Methods Sixty normal adult male Kunming mice were randomly divided into 3 groups（n=20 each）： control group,injury group,injury＋ resveratrol（10 μmol/L） group.A mouse model with non-lethal trauma Noble-Collip trauma apparatus.The techniques of 2D,M-mode and Doppler echocardiography were applied to evaluate the cardiac functions before injury as baseline（0 h） and at 5 time points of 12 h,24 h,36 h,48 h and 60 h after injury.Each mouse was numbered in order to monitor its cardiac function continuously.Results（1）There was no significant change in cardiac function change in control mice（P0.05）.（2）Compared with baseline,venous return decreased significantly after injury in injury group;Left ventricular end-diastolic volume（LVEDV） reached the lowest point at the time point of 12 h with a reduction of 46.1%（P0.05）;Notable compensatory changes in cardial function in injury mice were observed： heart rates reached a peak with an increase of 32.9%（P0.01） at the time point of 12 h after injury;Cardiac output was stable at each time point;Left ventricular ejection fraction（LVEF） and left ventricular fractional shortening（LVFS） of injury mice decreased by 16%and 20%（P0.05）;E/A ratio and E-wave deceleration time（EDT） also decreased by 36.8% and 29.1%（P0.05） respectively after injury.（3）Venous return also decreased in injury mice with resveratrol preconditioning（P0.05）;LVEDV reduced by 30.8%（P0.05）;LVEF and LVFS decreased by 8.4%and 17.7%（P0.05）,respectively;E/A ratio and EDT decreased by 24.6% and 24.1%（P0.05） respectively at the time point of 12 h.Conclusions Resveratrol preconditioning could notably ameliorate cardiac function in mice with stress-induced injury and enhance their cardiac compensation.