薄膜—均质法制备番茄红素脂质体的工艺研究

Preparation of lycopene liposome using membrane-homogeneous method

【目的】优化制备番茄红素脂质体的生产工艺,提高包封率,减小颗粒粒径,最终提高番茄红素的生物利用度。【方法】采用薄膜—均质法制备番茄红素脂质体,通过单因素试验和正交试验,以包封率作为主要指标,筛选出最佳制备工艺(胆固醇—卵磷脂比、番茄红素—卵磷脂比、旋转蒸发温度、磷酸缓冲液pH),并观察脂质体形态和粒径特征。【结果】薄膜—均质法制备番茄红素脂质体的最佳工艺为:胆固醇—卵磷脂比1:3,番茄红素—卵磷脂比1:20,旋转蒸发温度30℃,磷酸缓冲液pH7.0。在最佳工艺条件下,番茄红素脂质体的包封率为71.65%,平均粒径840nm。【结论】采用薄膜—均质法制备番茄红素脂质体具有工艺简单、重现性好,且脂质体包封率高、颗粒粒径小、稳定性较高等优点,可大规模用于实际生产。

[Objective]The experiment was conducted to enhance the bioavailability of lycopene by improving entrapment rate and reducing particle size. [Method]Lycopene liposome was prepared by membrane-homogeneous method. Entrapment rate was used as major index in single factor experiment and orthogonal test, where cholesterol/lecithin ratio, lycopene/lecithin ratio, rotating evaporation temperature and phosphate buffer solution were determined to obtain the opti- mal processing procedure. Furthermore, lipsome morphology and particle characteristics were recorded. [Result]The best processing parameters were observed as： cholesterol/lecithin ratio of 1：3, lycopene/lecithin ratio of 1：20, rotating evaporation temperature of 30 ℃ and phosphate buffer solution pH 7.0. Under the best technological conditions, entrapment rate of lycopene was 71.65% and the average particle size was 840 nm. [Conclusion]It was suggested that preparing lycopene lipsome using membrane-homogeneous method is simple and apt to controll. This method should be promoted in production due to its high entrapment rate, small particle and great stability.