血管内速溶支架的细胞毒性和植入实验研究

Cytotoxicity and Implant Test of Intravascular Quick-dissolving Stent

目的:检测一种新型的血管内速溶支架的生物相容性。方法:分别对血管内速溶支架进行细胞毒性实验和皮下植入实验。细胞毒性实验:速溶支架粉末与L929细胞接触培养,倒置显微镜观察形态,采用MTT(四唑盐)比色法量化细胞毒性,并进行毒性分级。皮下植入实验:在12只新西兰白兔右侧背部皮下植入血管内速溶支架,左侧作为空白对照,分别于植于支架后3、7、14、28 d,肉眼和镜下观察植入点炎症反应情况。结果:血管内速溶支架细胞毒性为0级。皮下植入后无炎症反应,局部组织无刺激。结论:该课题组黏合吻合血管用血管内速溶支架材料满足ISO 10993要求,具有良好的生物相容性。

Objective To test the biocompatibility of a new intravascular quick-dissolving stent.Methods Cytotoxicity and implant experiment were done to the stent separately.Cytotoxicity test：L929 cells were cultured with the stent powder.The cellular morphology was observed by inverted microscopy.The proliferation was examined using MTT method and cytotoxicity grade was evaluated.Implant test：intravascular quick-dissolving stents were implanted in the right back of 12 New Zealand white rabbits,the left as a blank control.The inflammatory reaction was examined by gross and microscopic observation 3,7,14,28 d after the implantation.Results The cytotoxicity grade of the stent powder was 0.Implant test showed intravascular quick-dissolving stents had no stimulation to the local tissue.Conclusion The cytotoxicity test and implant test of the new intravascular quick-dissolving stent accord with the standard of ISO-10993,which shows good biocompatibility.