玉屏风散对顺铂所致肝癌小鼠脏器氧化损伤的保护作用

The Protection of Yupingfeng Powder on Cisplatin Induced Oxidative Damage of Organs in Hepatocellular Carcinoma Mice

目的探讨玉屏风散对顺铂所致Hepa1-6肝癌小鼠脏器氧化损伤的影响。方法在C57BL/6小鼠右腋皮下接种2×106个Hepa1-6肝癌细胞建立肝癌小鼠模型,并随机分为模型组、顺铂组及玉屏风散加顺铂组,每组5只。顺铂组和玉屏风散加顺铂组每3天腹腔注射1次顺铂(2.5mg/kg),模型组相应注射生理盐水;同时玉屏风散加顺铂组每天灌胃玉屏风散水煎液(25g/kg),顺铂组和模型组相应灌服蒸馏水。给药观察14天。计算抑瘤率;称重肾脏、肝脏和肺重量,并计算脏器系数;测定组织中超氧化物歧化酶(SOD)活力及丙二醛(MDA)含量;进行病理学观察。结果与模型组比较,顺铂组及玉屏风散加顺铂组瘤重明显下降(P<0.05,P<0.01)。使用顺铂干预后,肝癌小鼠肾脏、肝脏均存在明显的氧化损伤,肺存在一定的氧化损伤;玉屏风散能明显降低顺铂干预的肝癌小鼠肝脏组织中MDA含量和SOD活力(P<0.05),明显增加肺组织中SOD活力(P<0.01)。病理观察结果显示了相同的作用趋势。结论扶正方药玉屏风散对顺铂所致肝癌小鼠脏器(肾脏、肝脏、肺)损伤具有保护作用,抗氧化是其机制之一。

Objective To study the effects of Yupingfeng Powder （YPFP） on cisplatin （DDP） induced oxidative damage of organs in hepatocellular carcinoma mice. Methods A total of 2 xl06 Hepa1-6 cells were inoculated sub- cutaneously into the right flank of 15 C57BL/6 mice to establish a mice model of hepatocellular carcinoma. Then the mice were randomly divided into three groups, i. e., the model group, the DDP group, and the DDP ＋YPFP group, 5 in each group. Mice in the DDP group and the DDP ＋YPFP group were intraperitoneally injected with DDP （2. 5 mg/kg）, once every three day for 2 weeks. Physiological saline was intrapedtoneally injected to mice in the model group. Mean- while, YPFP water decoction （25 g/kg） was given to mice in the DDP ＋YPFP group by gastrogavage once daily for 2 weeks. Corresponding distilled water was given by gastrogavage to mice in the DDP group and the model group. Four- teen days later, mice were sacrificed and the tumor inhibition ratio was calculated. The weights of kidneys, livers, and lungs were weighed and the organ coefficient calculated. The activities of superoxide dismutase （SOD） and the content of malondialdehyde （MDA） in the tissue were detected. The pathologic changes were observed. Results The tumor weight obviously decreased in the DDP group and the DDP ＋YPFP group when compared with the model group （P〈 0. 05,P〈0. 01 ）. Obvious oxidative damage existed in the kidneys and livers after induced by DDP. Oxidative damage also existed in the lungs to some extent. YPFP could obviously decrease the content of MDA and the activities of SOD in livers （P 〈0. 05）, and increase the activities of SOD in lungs （P 〈0. 01 ）. The pathologic changes showed the same effect trend. Conclusions YPFP could protect the organs （kidney, liver, lung） from the oxidative damage induced by DDP. Anti-oxidation is one of its mechanisms.