‘泰山早霞’苹果采后1-甲基环丙烯处理对其软化及相关基因表达的影响

The Regulation of 1-methylcyclopropene on Softening and Expression of Relevant Genes in‘Taishan Zaoxia’Apple

以‘泰山早霞’苹果成熟的果实为试材,于采收当天进行1–甲基环丙烯(1-MCP)处理,研究其对果实软化及相关基因表达的影响。结果表明,①1-MCP处理的果实在短期贮藏期间硬度均明显高于对照。②1-MCP处理后,乙烯释放速率、PG、PME、β-Gal、α-L-Af及LOX等细胞壁酶基因的表达均被明显抑制,在整个试验期内均有明显下降,尤其是在处理后1d,就分别比各自的对照下降了72.0%、72.1%、87.5%、81.8%、90.2%和16.7%,而1-MCP对AM和XET基因的表达没有明显作用。上述结果表明,苹果‘泰山早霞’品种属乙烯极敏感型,1-MCP对于延缓其果实软化具有明显作用,其果实软化可能是PG、PME、β-Gal、α-L-Af及LOX等多种基因协同作用的结果。

In order to study the molecular softening mechanism of‘Taishan Zaoxia’apple,the effect of 1-MCP on softening and expressions of genes related to softening in‘Taishan Zaoxia’were investigated. The results showed that：①The firmness of‘Taishan Zaoxia’fruit treated with 1-MCP were higher than the control fruit during the storage after harvest.②Ethylene production rate and the expression of PG,PME, β-Gal,α-L-Af and LOX genes of‘Taishan Zaoxia’fruit were inhibited significantly throughout the whole experimental period after treated with 1-MCP,especially in the first day after treatment. They decreased 72.0%,72.1%,87.5%,81.8%,90.2% and 16.7% than control respectively. But 1-MCP had no effect on expression of AM and XET genes. These results indicated that‘Taishan Zaoxia’apple variety was very sensitive to ethylene and 1-MCP could delay softening of‘Taishan Zaoxia’fruit obviously;The fruitsoftening may be caused by synergistic effect of PG,PME,β-Gal,α-L-Af and LOX genes.