IQGAP1在乳腺癌中的表达及意义

Expression and Significance of IQGAPl in Breast Cancer

目的:探讨IQGAP1(IQ motif containing GTPase activating protein 1)在乳腺癌组织中的表达及意义。方法:采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶(S-P法)染色法检测IQGAP1在乳腺癌组织及癌旁组织各110例中的蛋白表达水平。在正常乳腺上皮细胞系MCF-10A和乳腺癌细胞系MCF-7中开展IQGAP1表达调控实验。结果:IQGAP1在乳腺癌组织中的表达高于癌旁正常乳腺组织(P=4.23×10^(-6))。IQGAP1的表达与浸润性导管癌的肿瘤大小(P=0.006)相关,在肿瘤>2 cm组明显高于肿瘤≤2 cm组。未发现与淋巴结转移(P=0.870),临床分期(P=0.278),ER状态(P=0.412)和PR状态(P=0.248)相关。IQGAP1高表达与乳腺癌术后无病生存时间(p=0.008)和总生存时间(P=0.029)相关。在乳腺癌细胞系MCF-7中,IQGAP1降低导致细胞增殖活性显著下降。结论:IQGAP1可能在乳腺癌的发生发展过程中发挥重要作用。

Objective： To investigate the expression of IQGAPI in breast cancer tissues and their tumor-adjacent normal tissues and the effect of IQGAP 1 on the proliferation of breast cancer cells. Methods： A total of 110 breast invasive ductal cancer （ IDC ） and its adjacent normal tissues specimens were screened for the expression of IQGAP1 with streptavidin-perosidase （ S-P ） immunohisto-chemistry. IQGAP1 gene expression was regulated in MCF-7 and MCF-10A cell line. Results： The expression of IQGAP1 was higher in breast cancer tissues than in tumor-adjacent normal tissues （ P = 4.23 x 10 6 ）. The expression of 1QGAP1 was correlated with tumor size, and was significantly higher in tumor 〉 2 cm than in tumor ≤ 2 cm （ P = 0.006 ）, but was not correlated with lymphnode metastasis （ P = 0.870 ）, clinical staging （ P = 0.278 ）, expression of estrogen receptor （ ER ） （ P = 0.412 ） or progestogen receptor （PR） （ P = 0.248 ） in IDC. IQGAP1 high expression was associated with poor progression free time （ P = 0.008 ） and overall survival time （ P = 0.029 ） among breast cancer patients. The proliferation rate of MCF-7 cells was significantly decreased after knocking down IQGAP1 expression, Conclusion： IQGAP 1 may play important roles in the development of breast cancer.