单核细胞趋化蛋白-2518G／A基因多态性与冠心病的关系

MCP-1-2518G/A gene ploymorphisms and coronary atherosclerotic heart disease

目的研究单核细胞趋化蛋白-1（MCP-1）-2518G／A基因多态性对冠心病发病的影响。方法选取冠心病患者300例，正常对照组60例。冠心病患者分急性冠状脉综合征（ACS）组180例以及稳定型心绞痛（SAP）组120例。所有患者行冠状动脉造影（CAG）检查，采用Gensini评分。酶联免疫法测定MCP-1浓度，PCR-RFLP方法检测MCP-1-2518G／A位点多态性，研究MCP-1-2518G／A基因多态性与冠心病的相关性。结果（1）MCP-1-2518G／A基因多态性ACS组GG型基因和G等位基因频率分布较SAP组及正常对照组升高[GG型基因：ACS组（33．4％），SAP组（24．2％），对照组（15．0％）。P〈0．05；G等位基因频率：ACS组（51．1％），SAP组（40．4％），对照组（31．7％），P〈0．05]，ACS组AA型基因及A等位基因较SAP组及正常对照组降低[AA型基因：ACS组（31．1％），SAP组（43．3％），对照组（51．7％），P〈O．05；A等位基因频率：ACS组（48．9％），SAP组（59．6％），对照组（68．3％），P〈0．05]。（2）三种基因型间MCP．1浓度比较，GG型MCP-1浓度较AG型及AA型升高[GG型基因：（153±22）ng／L，AG型基因：（136±18）ng／L，AA型基因：（124±15）ng／L，（P〈0．05）]。（3）多元回归分析冠脉病变Gensini评分与低密度脂蛋白-胆固醇、空腹血糖、MCP-1GG型基因成正相关（P〈0．05），高密度脂蛋白-胆固醇与冠状动脉狭窄程度呈负相关（P〈0．05）。结论MCP-1-2518G／A基因多态性与冠心病严重程度相关；MCP-1-2518G／A基因多态G等位基因能通过增强MCP-1表达参与冠心病发病。

Objective To investigate the relationship between MCP-1-2518 G/A gene ploymorphisms and coronary heart disease （CHD）. Methods The 360 patients were divided into three groups to undergo the coronary angiography （CAG）： acute coronary syndrome （ACS） group （n=180）, stable angina pectoris （SAP） group （n=120） and control group （n=60））. The severity and extent of coronary lesions was analyzed by CAG and typified by means of Gensini coronary score system. Enzyme-linked immunosorbent assay was used to measure the MCP-1 concentration. PCR-RFLP way was used to measure MCP-1-2518 G/A gene polymorphisms. Results MCP-1 gene polymorphisms GG gene and G allelic gene was significantly higher in ACS group compared with the SAP group and the control group[GG gene： ACS（33.4%）, SAP（24.2%）, control group（15.0%）, P〈 0.05; G allelic gene： ACS（51.1%）, SAP（40.4%）, control group（31.7%）, P〈 0.05],and was significantly lower in ACS group compared with the SAP group and the control group[AA geue： ACS（31.1%）, SAP（43.3%）, control group（51.7%）, P〈0.05; A allelic gene： ACS（48.9%）, SAP（59.6%）, control group（68.3%）, P 〈 0.05]. The MCP-1 concentration in GG gene group was significantly higher than that in AG gene group and AA gene group[GG gene： （153 ＋ 22）ng/L,AG gene： （136 ＋ 18）ng/L, AA gene： （124 ＋ 15）rig/L, （P〈 0.05）]. The Gensini score of coronary artery lesions was positively correlated with low density lipoprotein cholesterol, free blood glucose, MCP-1-2518 G/A GG gene polymorpbisms （P 〈 0.05） and high density lipoprotein cholesterol was negatively correlated with stenotic degree of coronary artery.Conclusions MCP-1 gene ploymorphisms is positive regulators of coronary artery lesions. The G allelic ofMCP-1-2518 G/A polymorpbism can increase the MCP-1 expression than A allelic in cononary heart disease.