曲美他嗪对糖尿病心肌病大鼠心肌细胞纤维化的影响

Effects of Trimetazidine on Cardiac Fibrosis in Rats with Experimental Diabetic Cardiomyopathy

目的:探讨曲美他嗪对糖尿病心肌病大鼠心肌细胞纤维化的影响。方法:取Sprague-Dawley雄性大鼠30只,随机分为糖尿病心肌病组(D组,n=10),糖尿病心肌病曲美他嗪干预组(T组,n=10),另取健康大鼠10只,作为正常对照组(N组,n=10)。T组给予曲美他嗪[7.5mg/(kg.d)]灌胃,D组和N组给予等量蒸馏水灌胃,每日2次,共8周,每周称重调节药物用量。观察并比较3组试验动物心肌细胞纤维化的相关指标。结果:D组心肌羟脯氨酸水平(Hyp)(4.8±2.6)mg/g明显高于N组(2.5±1.0)mg/g和T组(2.7±1.7)mg/g,P均<0.05,且T组明显低于D组(P<0.05)。D组心肌间质胶原容积百分数(ICVF,5.6±2.1)明显高于N组(2.6±1.3)和T组(4.0±1.5),P均<0.05,且T组明显低于D组(P<0.05)。结论:曲美他嗪对抑制糖尿病心肌病大鼠心肌细胞纤维化有一定的作用。

Objective： To investigate the effects of trimetazidine on cardiac fibrosis in rats with experimental diabetic cardiomyopathy（DC）.Methods： DC was induced in male Sprague-Dawley rats by STZ intraperitoneally injection.Rats randomly received saline（n=10） or trimetazidine（7.5 mg per kg weight per day,n=10） for 8 weeks by gavage.Another 10 healthy rats were served as controls.Myocardial hydroxyproline content as well as interstitial collagen volume fraction（ICVF） were determined.Results： Myocardial hydroxyproline and ICVF were more significantly reduced in trimetazidine treated DC rats as compared to saline treated DC rats（all P0.05）.Conclusion： Trimetazidine could decrease the concentration of myocardial hydroxyproline and ICVF in diabetic cardiomyopathy rats.