期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

MicroRNA-29c在单侧输尿管梗阻大鼠中的表达及意义 被引量：5

Expression and significance of microRNA-29c in unilateral ureteral obstruction of rat
下载PDF
导出
摘要 目的 :观察microRNA-29c(miR-29c)在大鼠单侧输尿管梗阻(UUO)模型肾组织中的表达变化,探讨其是否参与肾间质纤维化及其可能机制。方法:高通量测序技术和实时荧光定量PCR检测miR-29c在UUO大鼠术后1 d和3 d肾脏组织中的表达情况。使用生物信息学预测miR-29c靶mRNA,对其靶mRNA进行Gene Ontology和pathway分析。结果:UUO大鼠术后1 d和术后3 d手术侧肾组织miR-29c表达下调,Col I mRNA和FN mRNA的表达升高。预测靶mRNA经分析后获得miR-29c有统计学意义的10个靶mRNA,其中6个编码细胞外基质蛋白。结论:miR-29c在大鼠UUO模型早期的肾组织中表达下调,可能通过促进细胞外基质堆积参与肾间质纤维化的发生发展。 Objective： To observe the change of expression pattern of miR-29c in kidney of unilateral ureteral obstruction（UUO） rats and discuss whether miR-29c take part in renal interstitial fibrosis（RIF） and its probable mechanism.Methods： High-throughput sequencing and real-time quantitative PCR analysed the expression of miR-29c in the kidney of UUO rats,which were killed 1 and 3 days after operation.The miR-29c targets were predicted by bioinformatics and then analysed with Gene Ontology analysis and pathway analysis.Results： The expression of miR-29c decreased,while the expression of Col I mRNA and FN mRNA increased in the surgical kidney of UUO rats.Ten miR-29c targets with statistical significance were obtained after analysis of thom,6 targets coded were extracellular matrix proteins.Conclusion： The expression of miR-29c decreased in the kidney of early UUO rat model,miR-29c may participate in RIF induced by UUO through promoting extracellular matrix accumulation.
作者 谢媚媚 章慧娣 黄慧雅 苏震 尤小寒 薛向阳
机构地区 温州医学院附属第一医院肾内科 温州医学院微生物学与免疫学教研室
出处 《温州医学院学报》 CAS 2012年第3期205-208,共4页 Journal of Wenzhou Medical College
基金 国家自然科学基金资助项目(30871179/C140405) 浙江省卫生高层次创新人才培养工程项目(浙卫发[2010]190号文件) 浙江省钱江人才计划项目(2011R10083)
关键词 miR-29c 单侧输尿管梗阻 肾间质纤维化 大鼠 miR-29c unilateral ureteral obstruction renal interstitial fibrosis rats
分类号 R742.1 [医药卫生—神经病学与精神病学]
  • 相关文献

参考文献19

  • 1Iwano M,Neilson EG.Mechanisms of tubulointerstitialfibrosis[J].Curr Opin Nephrol Hypertens,2004,13(3):279-284.
  • 2O’Donnell KA,Wentzel EA,Zeller KI,et al.c-Myc-regulatedmicroRNAs modulate E2F1 expression[J].Nature,2005,435(7043):839-843.
  • 3Bartel DP.MicroRNAs:genomics,biogenesis,mechanism,andfunction[J].Cell,2004,116(2):281-297.
  • 4Livak KJ,Schmittgen TD.Analysis of relative gene expres-sion data using real-time quantitative PCR and the 2(-DeltaDelta C(T))Method[J].Methods,2001,25(4):402-408.
  • 5Git A,Dvinge H,Salmon-Divon M,et al.Systematic compari-son of microarray profiling,real-time PCR,and next-gen-eration sequencing technologies for measuring differentialmicroRNA expression[J].RNA,2010,16(5):991-1006.
  • 6van Rooij E,Sutherland LB,Thatcher JE,et al.Dysregulationof microRNAs after myocardial infarction reveals a role ofmiR-29 in cardiac fibrosis[J].Proc Natl Acad Sci USA,2008,105(35):13027-13032.
  • 7Duisters RF,Tijsen AJ,Schroen B,et al.miR-133 and miR-30regulate connective tissue growth factor:implications for arole of microRNAs in myocardial matrix remodeling[J].CircRes,2009,104(2):170-178.
  • 8Thum T,Gross C,Fiedler J,et al.MicroRNA-21 contributesto myocardial disease by stimulating MAP kinase signallingin fibroblasts[J].Nature,2008,456(7224):980-984.
  • 9Ji J,Zhang J,Huang G,et al.Over-expressed microRNA-27aand 27b influence fat accumulation and cell proliferationduring rat hepatic stellate cell activation[J].FEBS Lett,2009,583(4):759-766.
  • 10Venugopal SK,Jiang J,Kim TH,et al.Liver fibrosis causesdown-regulation of miRNA-150 and miRNA-194 in hepaticstellate cells and their over-expression causes decreased stel-late cell activation[J].Am J Physiol Gastrointest LiverPhysiol,2010,298(1):G101-G106.

二级参考文献38

  • 1顾长江,张春辉,倪启超.乳腺癌骨转移的特异性相关因子[J].南通大学学报（医学版）,2009,29(3):192-194. 被引量：3
  • 2陈海蛟,王浩,王秋雁,张立,王国民.表皮生长因子信号通路对前列腺癌细胞DU145表面整合素α_5、β_1的调控[J].中华外科杂志,2006,44(14):966-969. 被引量：4
  • 3Vigneault F,Zaniolo K,Gaudreault M,et al.Contral of integrin genes in the eye[J].Prog Retin Eye Res,2007,26(2):99-161.
  • 4Humphries M J.Integrin structure[J].Biochem Soc Trans,2000,28(4):311-339.
  • 5Moser M,Leqate K R,Zent Y,et al.The tail of integrins,talin and kindlins[J].Science,2009,324 (5929):895-899.
  • 6Yano H,Mazaki Y,Kurokawa K.Roles played by a subset of integrin signaling molecules in cadherin-based cell-cell adhesion[J].The Journal of Cell Biology,2004,166(2):283-295.
  • 7Janssen M,Frielink C,Dijkgraaf I,et al.Improved tumor targeting of radiolabeled RGD peptides using rapid dose fractionation[J].Cancer Biother Radiopharm,2004,19(4):399-404.
  • 8Makrilia N,Kollias A,Manolopoulos L,et al.Cell adhesion molecules:role and clinical significance in cancer[J].Concer Invest,2009,27(10):1023-1037.
  • 9Takada Y,Ye X,Simon S.The integrins[J].Genome Biology,2007,doi:10.1186/gb-2007-8-5-215.
  • 10Lee H K,Lee J H,Kim M,et al.Insulin-like growth factor-1 induces migration and expression of laminin-5 in cultured human corneal epithelial cell[J].Invest Ophthalmol Vis Science,2006,47(3):873-882.

共引文献261

同被引文献54

  • 1聂秀,吴人亮.转化生长因子β在肾间质纤维化中的作用[J].国外医学（泌尿系统分册）,2005,25(3):409-412. 被引量：24
  • 2Nelson MM, Jones AR, Carmen JC, et al. Modulation of the host cell proteome by the intracellular apicomplexan para- site Toxoplasma gondii[J]. Infect lmmun, 2008, 76(2): 828- 844.
  • 3Saeij JP, Collerl S, Boyle JP, et al. Toxoplasma co-opts host gene expression by injection of a polymorphic kinase homologue[J]. Nature, 2007, 445(7125): 324-327.
  • 4Farh KK, Grimson A, Jan C, et al. The widespread impact of mammalian MicroRNAs on mRNA repression and evolu- tion[J]. Science, 2005, 310(5755): 1817-1821.
  • 5Cullen BR. Viruses and microRNAs[J]. Nat Genet, 2006, 38 Suppl: $25-30.
  • 6Lowery AJ, Miller N, McNeill RE, et al. MicroRNAs as prognostic indicators and therapeutic targets: potential effect on breast cancer management[J]. Clin Cancer Res, 2008, 14(2): 360-365.
  • 7Blader IJ, Manger ID, Boothroyd JC. Microarray analysis reveals previously unknown changes in Toxoplasma gondii- infected human cells[J]. J Biol Chem, 2001, 276(26): 24223- 24231.
  • 8Sinai AE Payne TM, Carmen JC, et al. Mechanisms under- lying the manipulation of host apoptotic pathways by Toxoplasma gondii[J]. Int J Parasitol, 2004, 34(3): 381-391.
  • 9Carmen JC, Hardi L, Sinai AP. Toxoplasma gondii inhibits ultraviolet light-induced apoptosis through multiple inter- actions with the mitochondrion-dependent programmed cell death pathway[J]. Cell Microbiol, 2006, 8(2): 301-315.
  • 10Kim JY, Ahn MH, Jun HS, et al. Toxoplasma gondii inhibits apoptosis in infected cells by caspase inactivation and NF- kappaB activation[J]. Yonsei Med J, 2006, 47(6): 862-869.

引证文献5

二级引证文献19

温州医学院学报
2012年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部