脾虚型复发口疮伴便秘患者肠道菌群的变化

Preliminary study on intestinal microecology of patients with recurrent aphthous ulcer and constipation

目的研究脾虚型复发口疮伴便秘患者肠道菌群的特征。方法选择肠道菌群中具有代表性的细菌共8种进行培养和计数,选择30例正常人为健康对照组,30例为脾虚型复发口疮便秘组,取当日首次定量大便作活性菌培养,检测两组相同菌种进行比较。结果脾虚型复发口疮患者便秘组的类杆菌、双岐杆菌、优杆菌、乳杆菌比对照组要显著减少(P<0.01或P<0.05),而消化链球菌、梭菌、葡萄球菌、肠杆菌则要比对照组显著增多(P<0.01或P<0.05),但肠球菌和酵母样菌在两组间无显著差异(P>0.05)。结论脾虚型复发口疮患者便秘与肠道菌群失调有关。

Objective To study the change of the intestinal bacterial flora in constipated patients with recurrent aphthous ulcer. Methods Eight bacteria in stool were cultured and the colony forming units were counted. Thirty constipated patients with recurrent aphthous ulcer in observation group and 30 cases in control group were studied. Bacteria in fresh stool were cultured and compared between the two groups. Results The number of bacteria per gram of stool between observation group and the control group were as follow： bac- teroides 7.85 ± 0.51 vs 10.31 ±0.62（P 〈 0.01）; bifidobacterium： 6.41 ± 0.61 vs 9.52 ± 0.71（P 〈 0.01）; pep- tostreptococcus 8.87 ± 0.47 vs 7.82 ± 0.67（P 〈 0.01）; fusobacterium： 8.05 ±0.57 vs 5.52 ± 0.23（P 〈 0.01）; lac- tobacillus： 6.15 ±0.32 vs 7.62 ±0.43（P 〈 0.01）; dominantbacteriaceas： 7.81±0.6 vs 9.02 ± 0.38（P 〈 0.01）; enteriobacterilli： 9.23 ± 1.85 vs 8.76±2.21（P 〈 0.01）; enterococcus： 7.50 ±1.65 vs 7.69 ± 1.82（P 〈 0.01）; staphylococci： 6.46 ± 1.72 vs 4.52 ± 1.61（P 〈 0.01）; yeast： 3.18± 1.85 vs 3.12 ±0.72（P 〉 0.05）. Conclusion Constipated patients with recurrent aphthous ulcer have imbalance of intestional flora which might contribute to constipated patients with recurrent aphthous ulcer.