草莓果实β-葡萄糖苷酶基因FaBG1克隆及成熟期表达分析

Cloning and transcription analysis of β-glucosidase gene FaBG1 during strawberry fruit ripening

为了探讨脱落酸合成相关酶β-葡萄糖苷酶FaBG1基因是否参与草莓果实成熟的调控,本研究以‘红颜’草莓着色期间的果实为试材,首先通过RT-PCR技术克隆β-葡萄糖苷酶FaBG1基因的编码区核苷酸序列。该1,845bp基因在基因组中以单拷贝形式存在,并编码1个含有615氨基酸的多肽。生物信息学分析表明,FaBG1含有3个跨膜区,在进化上高度保守。围绕果实着色期间FaBG1基因转录水平以及ABA含量分析表明,随着果实的着色增加,FaBG1转录水平和ABA含量都逐渐增加,半红后基因表达量逐渐降低,而ABA含量继续上升,至成熟时达到高峰。本研究结果表明FaBG1可能参与草莓果实的着色启动。

This study investigated whether β-glucosidase FaBG1 is involved in the regulation of strawberry fruit ripening. At first FaBG1 gene was cloned by RT-PCR technique with ＂Hongyan＂ red-coloring fruit as experiment material. The gene encodes a polypeptide containing 1, 381 amino acids, and harbors in genomic DNA with a single copy. Bioinformatics analysis showed that FaBG1 was evolutionarily conserved, and contained three putative transmembrane domains. Analysis of both transcription levels of FaBG1 and ABA contents during fruit red-coloring showed that coupled with fruit red-coloring, levels of both FaBG1 expression and ABA content increased gradually; after half of fruit red-coloring period, the former declined, in contrast, the latter continually increased and reached peak at fruit ripening. The results suggested the gene FaBG1 might participate in the onset of fruit red-coloring.