血管内皮细胞生长因子165基因转染干细胞促进组织工程化脂肪的存活

Stem cells transplanted with vascular endothelial growth factor 165 gene for improving the survival of tissue-engineered adipose

背景:体外构建工程组织时常因植入区血供不良或植入物建立血管化过程延长,造成种子细胞缺乏营养,代谢紊乱,增殖、分化及分泌功能受损甚至死亡,移植物最终失效。目的:观察血管内皮细胞生长因子165基因转染脂肪组织来源干细胞移植能否促进组织工程脂肪组织的血管新生。方法:利用腺病毒介导血管内皮细胞生长因子165基因体外转染人脂肪来源干细胞,然后与脂肪组织工程移植物混合移植于裸鼠背部(实验组),以移植脂肪来源干细胞+脂肪组织工程移植物(细胞组)、DMEM培养基+脂肪组织工程移植物(对照组)为对照。结果与结论:移植后3个月,实验组脂肪组织存活湿质量、脂肪组织血管密度高于细胞组与对照组(P<0.01,P<0.05),且细胞组均高于对照组。说明脂肪来源干细胞可促进组织工程脂肪组织的血管新生,提高存活水平,转染血管内皮细胞生长因子165基因后具有更强的促血管新生的作用。

BACKGROUND：During in vitro tissue engineering construction,poor blood supply in transplanted areas or delayed revascularization of implants leads to nutrition deficiency of seed cells,metabolism disorders,proliferation,differentiation and secretion dysfunction or even death,and at last causes the transplantation failure.OBJECTIVE：To investigate the feasibility of transfecting vascular endothelial growth factor 165（VEGF 165） gene into human adipose tissue-derived stem cells（ADSCs） for the neovascularization of tissue engineered adipose.METHODS：ADSCs were transfected by adenovirus-mediated VEGF 165 gene,and transfected ADSCs combined with collagen sponge were transplanted into the back of nude mice as experimental group.Non-transfected ADSCs with collagen sponge was used as cell group,and DMEM culture medium＋collagen sponge as control group.RESULTS AND CONCLUSION：Three months after transplantation,survival volume and capillary density of adipose tissue in the experimental group were higher than those of the cell and control groups,which in the cell group were higher than those of the control group（P 0.01,P 0.05）.It is indicated that ADSCs can improve the neovascularization and survival volume of tissue-engineered adipose tissues and VEGF 165 gene transfected ADSCs have a stronger ability of promoting the neovascularization of transplanted tissues.