脐血干细胞移植对帕金森病大鼠旋转行为的影响

Effect of human umbilical cord blood mesenchymal stem cells transplantation on rotational behavior of Parkinson's disease rats

背景:目前帕金森病的临床治疗还是以药物为主,细胞移植实验也多见于骨髓间充质干细胞,脐血来源干细胞移植能否改善帕金森病的旋转行为报道较少。目的:观察脐血间充质干细胞移植对帕金森病大鼠旋转行为的影响。方法:帕金森病模型大鼠随机分成实验组和对照组。实验组大鼠纹状体内植入用Hoechst33258标记的第4代脐血间充质干细胞,对照组注射PBS。此后每周腹腔注射阿扑吗啡以观察大鼠的旋转行为;并在移植后3,6,9周用免疫荧光双标法检测间充质干细胞的存活、迁移情况以及胶质纤维酸性蛋白、神经元特异性烯醇化酶、酪氨酸羟化酶和突触素的表达。结果与结论:移植脐血间充质干细胞后大鼠的旋转行为与对照组相比有明显改善(P<0.05);间充质干细胞可在大鼠脑内存活,随时间延长迁移范围扩大,分布于纹状体、胼胝体和皮质;胶质纤维酸性蛋白、神经元特异性烯醇化酶、酪氨酸羟化酶都有表达,突触素无表达。结果可见移植脐血间充质干细胞后能明显改善帕金森病大鼠旋转行为,有望成为治疗帕金森病的种子细胞。

BACKGROUND：To date,the clinical treatment of Parkinson＇s disease（PD） mainly depends on drug,and as for cell transplantation experiment,bone marrow mesenchymal stem cells（BMSCs） transplantation is the common method.The reports about whether umbilical cord blood mesenchymal stem cells（UCBMSCs） transplantation can improve the rotational behavior are rare.OBJECTIVE：To explore the effect of human UCBMSCs transplantation on rotational behavior of PD rats.METHODS：The PD rat models were divided into the experimental group（n=20） and the control group（n=20）.The fourth generation of MSCs were marked by Hoechst33258 and then transplanted into rat striatum in experimental group,and the rats in control group were given PBS.Apomorphine was injected intraperitoneally to examine the rotational behavior of the rats in two groups every week.Immunofluorescence double labeling method was used to identify the survival and migration of MSCs and the expression of glial fibrillary acidic protein（GFAP）,neuron specific enolase（NSE）,tyrosine hydroxylase（TH） and synaptophysin at the 3 rd,6 th and 9 th weeks.RESULTS AND CONCLUSION：The rotational behavior of PD rats in experimetal group was improved obviously compared with control group（P 0.05） after UCBMSCs transplantation.Part of MSCs could survive in brain tissue,and migrated to the striatum,callose and cortex with time prolonged and expressed GFAP,NSE and TH,but not expressed synaptophysin.UCBMSCs could obviously improve the rotational behavior of PD rats and were expected to be the seed cells to cure PD.