不同体外环境下舌癌干细胞标志CD44及ESA和CXCR4的表达

Expression of tongue cancer stem cells marker CD44,ESA and CXCR4 in different environments

背景:细胞生存的微环境与蛋白的表达密切相关,在不同的环境中癌干细胞标志的表达可能存在差异。目的:探讨癌干细胞标志CD44、ESA、CXCR4的表达是否会在不同的细胞生存环境中发生改变。方法:选取舌癌TCA8113细胞系,分别建立干细胞培养基、常规标准培养基以及在标准培养基中添加阿霉素的3种体外培养微环境,采用免疫组化及流式细胞术,检测不同培养环境中3种标志在舌癌细胞中的表达。结果与结论:免疫组化法检测显示,在所有培养环境中CD44、ESA强阳性表达,CXCR4在干细胞培养基环境中弱阳性表达,其余为阳性表达;流式细胞术检测CD44与ESA在所有培养环境中均高水平表达;与常规标准培养基环境比较,在干细胞培养基的微环境中CXCR4极低水平表达,将舌癌细胞从干细胞培养基转回常规标准培养基后,CXCR4的表达回升;在阿霉素干预的培养环境中,CXCR4表达水平升高。结果表明舌癌干细胞标志在不同体外微环境下的表达存在差异,需结合体内环境与肿瘤组织的表达情况来寻找癌干细胞标志。并且不同微环境模式可能富集不同性质的癌干细胞。

BACKGROUND：Cancer stem cells marker expression in different environments differ greatly because the microenvironment can impact the expression of proteins.OBJECTIVE：To discuss whether different cultures in vitro have influence on the expressions of cancer stem cells marker CD44,ESA and CXCR4.METHODS：Tongue cancer stem cells TCA8113 cell line was obtained.In vitro,three different microenvironments were established：stem cell culture medium,conventional standard medium and conventional standard medium supplemented with adriamycin.The expressions of three proteins in TCA8113 tongue carcinoma cells were detected by immunohistochemistry（IHC） and flow cytometry（FCM）.RESULTS AND CONCLUSION：In all microenvironments,IHC showed the expressions of CD44 and ESA were ＂＋＋＂.In stem cell culture medium and the other microenvironments,the expressions of CXCR4 were ＂±＂ and ＂＋＂ respectively.FCM found the expressions of CD44 and ESA were above 90% in all microenvironments;compared with conventional standard medium,the expression of CXCR4 was super low in stem cell culture medium,and recovery when tongue cancer cells reversed into conventional standard medium from stem cell culture medium;the expression of CXCR4 was increased in conventional standard medium supplemented with adriamycin.The expressions of cancer stem cell markers were various in different mircoenvironments;it needs to take various markers into account and combine with the experessions of markers on cancer cells in vivo to identify cancer stem cell markers.Different kinds of cancer stem cells may accumulate together by different microenvironments model.