纳米羟基磷灰石/羧甲基壳聚糖-海藻酸钠复合骨水泥与骨髓基质细胞的生物相容性

Biocompatibilities of nano-hydroxyapatite/carboxymethyl chitosan-sodium alginate compound bone cements and bone marrow stromal cells

背景:在前期的试验中,通过共沉淀法合成了纳米羟基磷灰石/羧甲基壳聚糖-海藻酸钠复合粉体,并与柠檬酸衍生物溶液调和制备出可生物降解、适当力学性能以及较好黏合强度的骨水泥。目的:验证纳米羟基磷灰石/羧甲基壳聚糖-海藻酸钠复合骨水泥材料对体外兔骨髓基质细胞黏附及增殖的影响,了解材料的生物相容性。方法:应用共沉淀法制备纳米羟基磷灰石/羧甲基壳聚糖-海藻酸钠复合材料作为骨水泥的固相粉体,将柠檬酸衍生物配制成溶液作为液相调和制备黏合性骨水泥。培养兔骨髓基质细胞,传代扩增后接种到材料上,体外继续培养;以细胞加入无材料的培养皿培养为对照。结果与结论:体外培养的兔骨髓基质细胞2d后呈梭形成纤维细胞样,生长良好。有材料实验组细胞数显著多于对照组(P<0.01)。扫描电镜下骨水泥材料具有良好的多孔网状结构,兔骨髓基质细胞伸出多个伪足样突起,紧密贴附在材料表面。两组细胞均保持持续增殖,2,4,6,和8d实验组增殖均显著快于对照组(P<0.01)。提示纳米羟基磷灰石/羧甲基壳聚糖-海藻酸钠复合骨水泥材料具有良好的生物相容性。

BACKGROUND： Nano-hydroxyapatite/carboxymethyl chitosan-sodium alginate （nano-HA/CMCS-SAL） composite powders were synthesized using the co-precipitation method in preliminary study. Biodegradable bone cements characterized as the appropriate mechanical properties and good adhesion strengths were prepared using the powders mixed with solution of citric acid derivatives. OBJECTIVE： To study the effects of nano-HA/CMCS-SAL compound bone cements on the attachment and proliferation of rabbit bone marrow stromal cells （BMSCs） in vitro and to explore their biocompatibilities. METHODS： Nano-HA/CMCS-SAL powders as solid phase of bone cements were prepared by the co-precipitation method. Bone cements were prepared using the liquid phase of citric acid derivatives solution. Rabbit BMSCs were seeded on the materials following passage and amplification for further culture in vitro. Cells cultured in the plates without materials were taken as controls. RESULTS AND CONCLUSION： Rabbit BMSCs grew well, and were spindle-shaped and fibroblast-like at 2 days of culture in vitro. The mean number of cells in the experimental group was obviously higher than that in the control group （P〈0.01）. Scanning electron microscopy observation： the bone cements had a good porous structure, and Rabbit BMSCs were attached to the material surfaces with cytoplasmic extensions and lamellipodia. The cells kept on a continued proliferation in both groups, and the proliferation of cells in the experimental group was obviously increased compared with the control group at 2, 4, 6 and 8 days of culture （P〈0.01）. It is indicated that the nano-HA/CMCS-SAL compound bone cements have a good biocompatibility.