超快效液相色谱与液面法测定榄香烯微乳的含量及包封率

Drug content and entrapment efficiency of elemene microemulsion detected by ultra-fast liquid chromatography and liquid surface method

背景：超快速液相色谱法具有快速、准确的优点,可以用来检测榄香烯微乳的含量。榄香烯微乳的包封率目前尚没有较好检测方法。目的：采用超快效液相色谱与液面法测定榄香烯微乳的含量及包封率。方法：应用超快速液相色谱法检测榄香烯微乳含量,根据榄香烯不溶于水而浮于水面的原理,设计液面法检测榄香烯微乳的包封率。结果与结论：色谱条件下β-榄香烯与辅料分离良好,β-榄香烯在3.94-27.58mg/L范围内线性关系良好。榄香烯微乳中β-榄香烯平均含量为（8.273±0.018）g/L,平均包封率为（99.81±0.24）%。提示超快速液相色谱和液面法可用于检测榄香烯微乳的含量和包封率,简单、快速且准确,榄香烯微乳包封率好。

BACKGROUND： Ultra-fast liquid chromatography is fast and accurate to detect content of elemene microemulsion. OBJECTIVE： To detect the drug content and entrapment efficiency of elemene microemulsion using ultra-fast liquid chromatography and liquid surface method. METHODS： The drug content of elemene microemulsion was determined by ultra-fast liquid chromatography. As elemene was insoluble in water and floated on water, liquid surface method was designed to detect the encapsulation efficiency of elemene emulsion. RESULTS AND CONCLUSION： It was better for β-elemene to separate from accessories under the chromatographic conditions, and β-elemene at a dose of 3.94-27.58 mg/L had a good linear relationship. The elemene microemulsion was characterized as （8.273 ± 0.018） g/L in content of β-elemene, and （99.81±0.24）% in average entrapment efficiency. The present study demonstrates that ultra-fast liquid chromatography and liquid surface method are simple, fast and accurate for determining the drug content and entrapment efficiency of elemene microemulsion. The elemene microemulsion has high entrapment efficiency.