釉基质蛋白组成及诱导细胞分化研究进展

Components of enamel matrix derivative and cyto-differentiation induction effects: A literature review

背景:研究表明,釉基质蛋白不仅能够诱导牙周组织再生,还对其他类型的细胞也有不同程度的作用效果。目的:综述釉基质蛋白的组成成分及其在诱导干细胞分化方面的研究进展,深入解析釉基质蛋白的生物模拟作用及其可能的机制。方法:应用计算机检索中国生物医学文献数据库,中国知网,万方、维普数据库1997-01/2011-12相关文献,检索词"釉基质蛋白,分化"限定文献语言种类为中文。同时计算机检索PubMed,EBSCOHOST数据库1997-01/2011-12相关文献,检索词"Enamelmatrixderivative,Emdogain?,differentiation",限定文献种类为英文。手工检索美国化学文摘数据库,荷兰医学文摘,进行文献初检。筛选后纳入38篇文章进行综述。结果与结论:釉基质蛋白组成成分复杂,包含有多种蛋白和类生长因子物质。釉基质蛋白具有生物模拟效果,对不同类型的干细胞具有不同程度的促进或抑制分化作用。提示釉基质蛋白将有可能不仅应用于牙周疾病的治疗,还可能应用于其他组织再生领域。

BACKGROUND： Enamel matrix derivative is a product derived from pig developing enamel which contains various enamel matrix proteins and derivatives. It is believed to act as a tissue-healing modulator which helps stimulate periodontal regeneration, biomineralization and bone induction. OBJECTIVE： To summarize the components of enamel matrix derivative and the effects of enamel matrix derivative on cyto-differentiation induction providing a basis for the further usage of enamel matrix derivative in tissue engineering research. METHODS： A computer-based online search of Chinese Biomedical Literature Analysis and Retrieval System, CNKI, Wanfang and VIP databases was undertaken for the related Chinese articles dated from January 1997 to December 2011 with the keywords of ＂enamel matrix derivative/enamel matrix protein, Emdogain, differentiation＂. Meanwhile, PubMed and EBSCO HOST databases were searched for the related English articles dated from January 1997 to December 2011 with the same keywords in English. In addition, Chemical Abstracts and Excerpta Medica were manually searched. Those experimental studies involving randomized, blind and controlled condition and observations, comparisons, academic summarization and case reports with no limitations were included. Those with unrelated research subjects, or repetitive studies were excluded. All articles were refined and finally 38 articles were remained for further analysis. RESULTS AND CONCLUSION： Enamel matrix derivative is the product derived from pig developing enamel which contains various enamel matrix proteins and growth factor-like materials. It is believed to act as a tissue-healing modulator which inhibits or stimulates differentiation of various stem cells. It suggests that enamel matrix derivative can be used not only in periodontal regeneration, but also in other tissue engineering fields.