柴胡皂苷a对难治性癫痫大鼠痫性发作的影响

Effect of saikosaponin-a on epileptic seizures in refractory epilepsy rats

目的探讨柴胡皂苷a(SSa)对匹罗卡品致难治性癫痫模型大鼠痫性发作的影响。方法将制备成功的48只SD难治性癫痫大鼠模型随机分为模型组(B)、丙戊酸钠组(C)、柴胡皂苷a低剂量组(D)和高剂量组(E)4组,每组各12只,另有空白对照组(A)12只。A、B组给予等量生理盐水,C组用量为200mg/kg,D、E组用药浓度依次为1.09mg/kg、2.18mg/kg,A、B、C组灌胃给药,D、E组腹腔注射给药。分别观察给药4周、8周后痫性发作次数、平均发作时间及发作级别的变化。结果痫性发作方面,A组无痫性发作,B组大鼠痫性发作明显(P>0.05),C、D、E组大鼠痫性发作均有不同程度的减轻(P<0.05),E组大鼠痫性发作减轻最明显。结论柴胡皂苷a能减轻难治性癫痫大鼠的痫性发作,有明显的抗癫痫作用。

Objective To investigate the effect of saikosaponin-a （SSa） on epileptic seizures of refractory epilepsy rats induced by pilocarpine. Methods Except for the control group （A）, 48 Sprague-Dawley refractory epilepsy model rats were randomly divided into 4 groups, namely the model group （B）, sodium valproate group （C）, low and high dose of SSa group （D and E）.Group A and B were given with the same amount of saline by lavage,and group C was administrated with sodium valproate 200 mg/kg.Group D and E were given intraperitoneal injection of 1.09 mg/kg and 2.18 mg/kg SSa, respectively. At 4 weeks and 8 weeks after treatment, the frequency, average time and the degree of epileptic seizure in RE rats were evaluated. Results There was no epileptic seizure in group A,but more epileptic seizure was observed in group B（P0.05）. Epileptic seizures were decreased in group C, D and E when compared with group B （P0.05）. The reduction of epileptic seizure was most apparent in group E. Conclusion SSa has a prominent antiepiletic effect to decrease epileptic seizure in rats.