siRNA抑制PPARγ表达对绒癌细胞JEG-3侵袭力的影响

Influence of siRNA-mediated PPARγ gene knockdown on invasion ability of chorioepithelioma JEG-3 cells

目的:采用小分子干扰RNA(siRNA)技术特异性地抑制绒癌细胞株JEG-3中过氧化物酶体增殖物激活受体γ(PPARγ)的表达,研究PPARγ影响JEG-3细胞侵袭力的机制。方法:将实验设为实验组(转染PPARγ基因的特异性siRNA)、阴性对照组(转染阴性对照siRNA)和空白对照组(不转染任何siRNA片段,其它试剂与另两组相同),采用实时定量PCR技术从mRNA水平检测PPARγ及黏蛋白-1(MUC1)的表达;利用Transwell侵袭实验观察siRNA转染JEG-3细胞后细胞侵袭力的变化。结果:siRNA使PPARγmRNA的表达水平下调了(75.0±0.8)%(P<0.05),MUC1 mRNA的表达水平下调了(65.0±1.3)%(P<0.05),JEG-3细胞侵袭Matrigel的能力显著增强(P<0.05)。结论:PPARγ表达水平的下降能相应下调MUC1的表达水平,并增强滋养细胞的侵袭能力,推测PPARγ可能通过调节MUC1基因影响滋养细胞的侵袭能力,从而参与了子痫前期等病理妊娠的发生。

AIM： To investigate the effect of peroxisome proliferator - activated receptor γ （PPARγ） on the in- vasion ability of trophoblasts. METHODS： A segment of PPARγ/ siRNA was synthesized. Three groups were designed： experiment group, negative control group and blank control group. The PPARγ/siRNA was transfected into JEG -3 ceils. Real - time quantitive PCR was used to detect the mRNA levels of PPARγ/and mucin - 1 （ MUC1 ）. The Transwell culture inserts were used to detect the invasion ability of JEG- 3 cells 24 h after treated with PPAR,/siRNA. RESULTS： Mter transfected with PPAR＇y siRNA, the mRNA expression levels of PPARγ/and MUC1 were significantly depressed by （75.0 ± 0.8） % and （65.0 ± 1.3 ） % （ P 〈 0. 05 ）, respectively, and the invasion ability of JEG - 3 cells was significantly strengthened （P 〈 0. 05 ）. CONCLUSION： The depression of PPAR7 gene down - regulates the expression of MUCI, and affects the invasion ability of trophoblasts, indicating that PPARγ may regulate the invasion of trophoblasts by MUC1 and involve in the development of placental defects such as preeclampsia.