酸性成纤维细胞生长因子复方保存液对大鼠肝脏低温保存的作用

Protective effects of aFGF compound solution on rat liver in hypothermic preservation

目的:探讨人酸性成纤维细胞生长因子(aFGF)对离体大鼠肝脏低温保存的效果。方法:24只Wistar大鼠随机分为高渗枸橼酸盐腺嘌呤(HCA)液对照组和aFGF复方保存液(HCA液中含aFGF14-15440μg/L)实验组,分别用HCA液和aFGF复方保存液进行肝脏离体灌注,并低温保存肝脏。12和24 h后,测定肝脏保存前后的重量、肝组织丙二醛(MDA)含量及肝保存液中丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)活性的变化,并检查肝组织病理学改变。结果:离体肝脏低温保存24 h后,aFGF复方保存液组肝脏重量差、ALT、AST和MDA水平均明显低于HCA对照组(P<0.05);肝脏病理学检查结果显示,aFGF复方保存液组肝组织损伤明显减轻。结论:aFGF复方保存液对肝脏的低温保存效果较单纯HCA液更明显,其机制可能与aFGF参与抑制细胞肿胀和抗氧化损伤作用有关。

AIM： To investigate the protective effects of human acidic fibroblast growth factor （aFGF） on hy- pothermic preservation of rat liver. METHODS： Twenty - four Wistar rats were randomly divided into 2 groups （ n =6） ： hypertonic citrate adenine （HCA） solution control group and experimental group （aFGF compound solution, containing 40 μg/L aFG14-154 in HCA solution）, The isolated livers were preserved in corresponding solution for 12 h or 24 h, and the content of malondialdehyde （MDA）, the activity of alanine aminotransferase （ALT） and aspartate aminotransferase （ AST）, and the changes of liver weight in the 2 groups were analyzed. The histopathological changes of the livers were also examined. RESULTS： Compared with HCA control group, the levels of ALT, AST and MDA and the changes of liver weight were significantly decreased in experimental group （24 h）, and the changes of liver histopathology were obviously alleviated in experiment group. CONCLUSION： aFGF can reduce rat hepatic injury. The protective effects of aFGF may be related to attenuating cell swelling and improving the capacity of anti - oxidative damage in the liver.