摘要
目的 比较增生性瘢痕与正常皮肤组织的蛋白质组表达差异,筛选出增生性瘢痕的特异蛋白质。方法 以中山大学附属第一医院烧伤外科2010年1月至5月8例增生性瘢痕患者的增生性瘢痕组织和3例整形外科患者正常躯干皮肤为研究对象,运用蛋白质组学技术、双向凝胶电泳对比分析增生性瘢痕组织和正常皮肤组织中蛋白表达差异,选择差异蛋白点进行MALDI.TOF/TOF质谱分析和生物学信息分析。结果 获得重复性较好的双向电泳荧光染色图谱,增生性瘢痕和正常皮肤组织凝胶电泳图谱中平均蛋白点分别为2975和3053,对其中表达差异超过4倍的蛋白点进行质谱分析和数据库检索,共鉴定出24种蛋白质,其中上调蛋白有11种,下调蛋白有13种。结论成功地建立增生性瘢痕的双向电泳荧光染色图谱,鉴定出增生性瘢痕组织与正常皮肤组织间的差异蛋白质表达。
Objective To screen specific proteins in patients with hypertrophic scar via comparing the distinction in proteomics to normal skin. Methods Hypertrophic scar tissues ( 8 patients with hypertrophic scar ) and normal cutaneous tissues (3 patients underwent trunk plastic surgery) were collected from Department of Burns Surgery, The First Affiliated Hospital of Sun Yat-sen University, between January 2010 and May 2010. The distinction in proteomics between hypertrophic scars and normal cutaneous tissues was compared via two- dimensional gel electrophoresis. The protein spots with differential expression were subjected to MALDI-TOF/TOF mass spectrometry and bio-information analysis. Results Two-dimensional electrophoresis fluorescent staining atlas with high repeatability was obtained, showing an average of 2975 protein spots in hypertrophic scar and 3053 in normal cutaneous tissue. Further analyses on the spots with a minimum of 4-fold distinction identified 24 proteins (11 up-regulated and 13 down-regulated) via mass spectrometry and database searching. Conclusion Successful establishment of two-dimensional electrophoresis fluorescent staining atlas is helpful to identification of differential proteins between hypertrophic sear and normal skin.
出处
《中华生物医学工程杂志》
CAS
2012年第2期89-92,共4页
Chinese Journal of Biomedical Engineering
基金
国家自然科学基金资助项目(30973128)
广东省自然科学基金(8151008901000082)
关键词
瘢痕
增生
蛋白质组学
电泳
凝胶
双向
Cicatrix
Hyperplasia
Proteomics
Electrophoresis, gel, two-dimensional