红花黄色素抗氧化活性研究

Study on the antioxidation of safflower yellow

通过考察红花黄色素(SY)及其主要化学成分对羟基自由基介导2-脱氧核糖氧化降解的抑制作用,以及对1,1-二苯基-2-苦肼基自由基(DPPH.)的清除能力,探究SY的体外抗氧化活性及其抗氧化的主要有效成分。通过Fenton反应产生羟基自由基,用紫外分光光度计检测了SY及其主要化学成分对2-脱氧核糖降解的抑制作用和对DPPH.的清除能力,同时对红花黄色素B(SYB)抑制2-脱氧核糖降解的作用机制做了初步研究。结果表明SY抑制Fenton反应对2-脱氧核糖的氧化降解的IC50为256.79μg/mL,对DPPH.清除的IC50值为27.15μg/mL。羟基红花黄色素A(HSYA)和SYB是SY中抗氧化的两个有效成分,抑制2-脱氧核糖的氧化降解的IC50分别为220.68μg/mL和207.01μg/mL;对DPPH.清除的IC50值分别为55.81μg/mL和41.25μg/mL。SYB对2-脱氧核糖氧化降解的抑制作用机理研究表明,SYB除了对Fenton反应产生的羟基自由基具有直接清除作用外,又可通过与Fe2+离子的络合作用而阻断Fenton反应产生羟基自由基。由此可知SY具有明显的体外抗氧化活性,SYB和HSYA为其主要抗氧化活性成分。

To explore the antioxidant activity of Safflower yellow（SY） and its main active ingredient,the inhibition of 2-deoxyribose degradation induced by hydroxyl radical and the ability of scavenging DPPH. was examined. Hydroxyl radical was produced through the Fenton reaction. The protection ability of SY and its main chemical components on 2-deoxyribose degradation induced by hydroxyl radical, and the ability of scavenging DPPH. were examined in vitro by UV spectrophotometer. At the same time, the mechanism of Safflower yellow B （SYB） inhibiting the degradation of 2-deoxyribose had been studied. Results showed that SY could inhibit the degradation of 2-deoxyribose induced by hydroxyl radical. SY could also eliminate DPPH. effectively. The ICso was 256. 79 μg/mL and 27. 15μg/mL respectively, and both of them showed a dose-effect relationship. Hydroxy safflower yellow A （HSYA）and SYB were two main active compounds in SY. The ICso of inhibition 2-deoxyribose degradation was 220. 68 μg/mL and 207.01 μg/mL, and the ICso of scavenging DPPH-was 55. 81 μg/mL and 41.25 μg/mL respectively. The results of mechanism test showed that,apart from direct scavenging the hydroxyl radicals produced by the Fenton reaction, SYB could also chelated with iron ions and block Fenton reaction. So SY had a significant antioxidation activity, while SYB and HSYA were main ingredients in SY.