摘要
目的:探讨人参皂苷Rg1经PEG修饰前后在大鼠离体胃中的稳定性。方法:取SD大鼠,禁食18 h后,随机分为Rg1组和PEG-Rg1组,准确吸取Rg1胃灌注液和PEG-Rg1胃灌注液注射到离体胃内,37℃恒温振荡,不同时间点取样,采用UPLC对样品中指标成分人参皂苷Rg1进行含量测定,观察比较人参皂苷Rg1与PEG-Rg1在离体胃中的稳定性差异。结果:Rg1在大鼠离体胃中的稳定性差,2 h时测得Rg1为26.8%,降解73.2%,PEG-Rg1在大鼠离体胃中的稳定性提高,2 h时测得Rg1仍有81.8%,仅降解18.2%。结论:PEG修饰之后可以提高人参皂苷Rg1在胃中的稳定性,可以改善修饰之前游离的人参皂苷Rg1在胃中容易降解,稳定性差等问题。
Objective: To study the stability of ginsenoside Rg1 before and after being modified by PEG ( PEG-Rg1 ) in isolated rat stomachs. Method: SD rats, after 18 h of fasting, were randomly divided into the Rg1 group and the PEG-Rg1 group. Rg1 stomach perfusion fluid and PEG-Rg1 infusion fluid were accurately extracted and injected into their stomachs in vitro, with oscillation at 37 ℃. Samples were taken in different time points and contents of ginsenoside RgI were determined by UPLC to observe and compare the stability of ginsenoside Rg1 and PEG-Rg1 in rat stomachs in vitro. Result: Rg1 in rat stomachs showed poor stability, Rg1 was measured to be 26.8% in 2 h, with degradation of 73.2%. Its stability in PEG-Rg1 was improved in rat stomachs, Rg1 was measured to be 81.8% in 2 h, with degradation of only 18.2%. Conclusion: PEG-modified ginsenoside Rg1 can enhance the stability of ginsenoside Rg1 in stomach and improve degradation and poor stability of ginsenoside Rg1 in stomach.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第10期1378-1382,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30873445)