黑果枸杞花色苷对小鼠动脉粥样硬化的影响

Effect of Lycium ruthenicum anthocyanins on atherosclerosis in mice

目的:研究黑果枸杞花色苷对小鼠动脉粥样硬化(AS)的影响。方法:以正常小鼠作空白对照,将高脂血症模型小鼠分为高脂模型组、黑果枸杞花色苷低、中、高剂量组和辛伐他汀药物对照组,进行不同灌胃处理,酶法检测各组小鼠血脂指标;苏木素伊红(HE)染色观察主动脉、心脏及肝脏组织形态学变化,并对肝脏相关指标进行测定。结果:与高脂组对照,花色苷各剂量组小鼠血清的总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)和动脉粥样硬化指数(AI)水平均显著降低(P<0.05),高密度脂蛋白胆固醇(HDL-C)水平有高于模型组的趋势。肝脏的总抗氧化能力(T-AOC),谷胱甘肽过氧化物酶(GSH-PX)、脂蛋白酯酶(LPL)均显著升高(P<0.05),丙二醛(MDA)极显著下降(P<0.01);花色苷各剂量组主动脉斑块面积占总面积的百分比显著低于模型组(P<0.05);主动脉、心脏和肝脏的病变程度明显轻于高脂组,其中,中剂量组与辛伐他汀组类似。结论:黑果枸杞花色苷可以干预小鼠AS的形成,同时降低小鼠血脂水平。

Objective： To study the effect of Lycium ruthenicum anthocyanins on atherosclerosis （AS） in mice. Method： Normal mice were taken as the control group, and hyperlipemia mice were divided into the model group, Lycium ruthenicum anthocyanins low, medium and high dose groups, and the simvastatin drug control group. After the oral administration, blood lipid indicators were detected by enzymatic analysis. The histomorphological changes in aortas, hearts and livers were observed, and liver-related indicators were determined by using hematoxylin-eosin （HE） staining. Result： Compared with the high-fat group, L. ruthenicum anthocyanins low, medium and high dose groups showed significant decrease in total cholesterol （TC）, triglyceride （TG）, low-density lipoprotein cholesterol （LDL-C） and atherosclerotic index （AI） （ P 〈 0. 05 ）. However, high-density lipoprotein cholesterol （HDL-C） level showed a trend of higher than the model group. Liver＇ s total antioxidant capacity （T-AOC） , Glutathione peroxidase （GSH-PX） , lipoprotein lipase （LPL） were significantly increased （P 〈0. 05）, malondialdehyde （MDA） was markedly decreased （P 〈0. 01 ）; the percentage of aortic plaque area of each anthocyanins dose group in the total area was significantly lower than the model group （ P 〈 0. 05 ） ; severity of aorta, heart and liver were significantly lighter than the high-fat group. But the media dose group was similar with the simvastatin group. Conclusion： L. ruthenicum anthocyanins can interfere the formation of AS, while lowering blood lipid levels in