MMP9 mRNA与MMP13 mRNA在小鼠长骨发育中的表达

MMP9 mRNA,MMP13 mRNA expression in the development of long bone in mice with in situ hybridization

目的测定MMP9 mRNA在小鼠胚胎和成年期长骨发育阶段的表达,并与MMP13 mRNA的表达相比较,探讨MMP9,MMP13在长骨发育中的作用。方法应用原位杂交技术,用同位素标记探针显示小鼠不同发育阶段长骨组织中MMP9 mRNA与MMP13 mRNA的表达。结果小鼠胚胎15.5d时在胫骨原发性骨化中心及软骨壳外侧有大量MMP9 mRNA表达,MMP13 mRNA的表达局限在原发性骨化中心及软骨的肥大软骨细胞末端。在1月龄小鼠胫骨切片中,MMP9 mRNA主要在骨与软骨交界处表达,MMP13 mRNA在骨与软骨交界处与干骺端小梁骨的表面均有表达。结论 MMP9在小鼠胚胎期长骨发育的基质降解和骨化过程中起重要作用,是软骨吸收和软骨内骨化的另一个标记物。1月龄小鼠MMP9 mRNA仅在骨与软骨交界表达处,不参与骨基质的降解和转换。

Objective Through measuring the MMP9 mRNA expression in long bone development in the embryo and adult mice,comparing with MMP13 mRNA expression,to further investigate the function of MMP9,MMP13 in long bone development.Methods Using in situ hybridization,with the isotope labeled probe,showing the MMP9,MMP13 mRNA expression in mice tissue section in different period of long bone development.Results MMP9 mRNA expression were found in the primary ossification center and subperiosteum in 15.5days embryo.MMP13 mRNA expression were seen in the end of the growth plate and primary ossification center in 15.5days embryo.In one month mice tissue section,the MMP9 mRNA expression located were at the junction of the bone and cartilage.MMP13 mRNA was not only expressed at the junction of the bone and cartilage,but also expressed at the epiphyseal bone surface.Conclusion MMP9 plays an important role in the degradation of cartilage matrix and ossification.There is no expression in the hypertrophic chondrocyte.It was a different marker for cartilage resorption and endochondral ossification.In one month mice,MMP9 mRNA was only expressed in osteochondral junction.There is no expression in the epiphyseal bone surface as MMP13.It possibly has no function in the bone degradation and turnover.