卵巢癌中C-erbB_2、p53基因表达及临床意义

Expression and clinical significance of C-erbB_2 and p53 in ovarian cancer

目的:探讨卵巢癌中C-erbB2基因、p53基因的表达情况及p53蛋白表达与PCNA的相关性。方法:对比2008年1月～2009年1月应用免疫组化SP三步法检测的40份卵巢癌组织、10份正常卵巢组织中C-erbB2基因、p53基因的表达情况及PCNA计数。结果:卵巢癌组织中存在C-erbB2基因与p53基因的异常表达,阴性率显著高于正常卵巢组(P<0.01),p53蛋白表达阳性与阴性两组间PCNA计数差异有统计学意义,阳性组高于阴性组(P<0.05)。结论:C-erbB2基因、p53基因的异常表达与卵巢癌的发生有关,其异常表达强度与肿瘤的分化及患者的预后有关,临床联合检测C-erbB2基因、p53基因的表达情况及PCNA计数,有助于准确判断卵巢癌的恶性程度及患者的预后。

Objective. To explore the gene expression of C-erbB2 and p53 in ovarian cancer and correlation between the protein expression of p53 and PCNA. Methods. The gene expression of C-erbB2 and p53 and the count of PCNA in 40 tissues of ovarian cancer and 10 normal ovarian tissues which were collected from January 2008 to January 2009 were compared by SP three-step method of immunohistochemistry. Results： Abnormal gene expression of C-erbB2 and p53 was present in the tissues of ovarian cancer. The negative ratio was significantly higher than that of the normal group （P〈0.01）. There was a significant difference in the count of PCNA between the two groups of positive and negative protein expressions of p53. The count in the positive group was higher that in the negative group （P〈0.05）. Conclusions： It can be concluded that the abnormal gene expression of C-erbB2 and p53 is associated with the occurrence of ovarian cancer and its intensity is also related with the tumor differentiation and patients＂ prognosis. Combined detection of C-erbB2 and p53 and the count of PCNA will help to accurately diagnose the malignant degree of ovarian cancer and patients＂ prognosis.