运动时间及强度对慢阻肺患者细胞因子的影响

Influence of doing exercise for different length of time and with different degree of intensity on serum cytokine levels in COPD patients

目的:探讨缓解期COPD患者太极拳不同运动时间、强度对IL-1、IL-6、TNF-α血清细胞因子的影响。方法:选择临床缓解期的COPD患者140例,将55例随机分组进行为期12周不同强度太极拳运动训练,同时将55例随机分组进行为期12周不同时间太极拳运动训练,并将30例作为无太极拳干预的对照组,每组运动训练前后均采用双抗体夹心ABC-ELISA法检测患者血清中细胞因子值。结果:四组太极拳运动组IL-1、TNF-α细胞因子的下降程度与对照组相比有显著统计学意义(P<0.05)。太极拳运动中等强度组IL-1、TNF-α下降的幅度显著大于低等强度组(P<0.05),60分钟组IL-1、TNF-α下降的幅度显著大于30分钟组(P<0.05),两组实验中IL-6的下降幅度无统计学意义(P>0.05)。结论:COPD患者太极拳运动随着时间的延长而达到中等强度,这种中等强度的太极拳运动对IL-1、TNF-α致炎因子有下调作用,能降低缓解期COPD患者的全身性炎症反应,但对IL-6水平下降的敏感性不强。

Objective： To evaluate influence of doing Taijiquan at different times and with different intensities on IL-1、IL-6、TNF-α levels in patients with COPD of stable phase.Methods： A total of 140 COPD cases of remission stage were enrolled and randomly divided into different groups in this study.Fifty-five cases were randomly divided into sub-groups doing Taijijuan for different length of time,for consecutive 12 weeks;another 55 cases doing the exercise with different intensity,also for 12 weeks;other 30 cases were assigned as negative control group.Sandwich ABC-ELISA assay were applied to determine the serum IL-1,IL-6,and TNF-α levels of each group before and after the training.Results： Decrease in serum IL-1,TNF-α levels of the four trial groups doing Taijiquan were more significant（P0.05） than that of the control group.However,within the subgroups,the level of serum IL-1,TNF-α in the group doing Taijiquan with medium intensity fell more significantly than the group doing the exercise with low intensity（P0.05）;Similarly,serum IL-1,TNF-α levels in the group doing Taijiquan for sixty-minutes per time decreased more significantly than the group doing Taijiquan for thirty-minutes（P0.05）.No significant difference in the decrease of IL-6 level was found（P0.05） among the trail groups.Conclusion： With the extension of time,the exercise of Taijijuan can achieve medium intensity,which can reduce the proinflammatory cytokines levels of IL-1,TNF-α and relieve the chronic inflammation reaction in COPD remission stage,but it has little effects on regulating serum IL-6 level.