新型凝血酶电化学传感器的制备

The Preparation of the Novel Electrochemical Biosensor for Thrombin Detection

制备了金纳米粒子-核酸适体/凝血酶/巯基六醇修饰的金电极,测试了该修饰电极检测凝血酶含量的性能指标.利用电化学技术研究了电极的构造对检测凝血酶含量的影响,探究了最佳实验条件.研究结果表明,在最佳的实验条件下构筑的修饰电极检测凝血酶的线性范围为1.0~10.0 nmol/L,检测限为0.2 nmol/L,并模拟了实际样品进行检测.该方法简便迅速,有较高的检测灵敏度、较好的重复性和抗干扰性.

This work prepared an AuNPs-aptamer/thrombin/6-mercapto-1-hexanol modified Au electrode and measured some parameters for the electrochemical detection of thrombin.Electrochemical technique was used to investigate the construction of the modified electrode and its effects on thrombin detection and explored the optimal experiment conditions.The results indicated that the modified electrode showed a good linear relationship between the peak current and the concentration of thrombin in the range of 1.0~10.0 nmol/L with a detection limit of 0.2 nmol/L.And the modified electrode was applied in the detection of analogous thrombin samples.It was found that the resulted sensor had high sensitivity,good reproducibility and anti-interference.