开口箭醇提物抗慢性咽炎活性研究

Tupistra Alcohol Extract Activity of Anti-Chronic Pharyngitis

观察开口箭醇提物治疗慢性咽炎的大鼠血清IL-1β及TNF-α等指标变化,以进一步探讨其治疗机理.将48只Wistar大鼠随机分为正常组、模型组、开口箭醇提物低、中、高剂量组和阳性组(西瓜霜组),每组8只,采用浓氨水喉头喷雾诱发的大鼠急性咽炎模型,于第40～47天分别用喉头喷雾器给大鼠咽部喷蒸馏水、低剂量开口箭醇提物(0.16g/kg)、中剂量开口箭醇提物(0.24g/kg)、高剂量开口箭醇提物(0.32g/kg)、西瓜霜含片液(1.40g/kg).实验第47d取6组动物的股静脉血检测白细胞和粒细胞数目、IL-1β及TNF-α含量;同时,取咽部粘膜及粘膜下组织分成两份分别进行病理形态学观察.结果显示开口箭醇提物均能有效降低模型大鼠血液白细胞和粒细胞数目、IL-1β及TNF-α含量,改善模型大鼠咽部组织病理形态.说明开口箭醇提物在缓解咽部炎性症状、改善咽部病理组织形态方面,具有独特优势.

In order to investigate the mechanism of Tupistra alcohol extract in treating chronic pharyngitis,effects of Tupistra alcohol extract on IL-1β and TNF-α in the serum were observed in the rats with chronic pharyngitis.48 Wistar rats were divided into 6 groups,namely the normal control group,large-dose Tupistra alcohol extract group,small-dose Tupistra alcohol extract group,model control group,Xiguashuang group,and each group consisted of 8 rats.The rat model of chronic pharyngitis was induced by spraying the larynx with strong ammonia water.Distilled water,large dose of Tupistra alcohol extract solution（0.32g/kg）,middle dose of Tupistra alcohol extract solution（0.24g/kg）,small dose of Tupistra alcohol extract solution（0.16g/kg） and Xiguashuang solution（1.40g/kg） were respectively given to the animals in each corresponding group.On the 47th day of the experiment,the levels of IL-1β and TNF-α in the serum were detected in the blood of the femoral vein of the animals in the 5 groups,respectively,and at the same time,the pharyngeal mucosal and submucosal tissues of the animals were also examined pathomorphologically.The results showed that both large dose and small dose of Tupistra alcohol extract could effectively decrease the percentage of blood lymph cells and the levels of IL-6 and TNF-α in the serum,and improve the pathologic picture of the pharyngeal tissues in the model animals.