分析老年矽肺结核病的耐药水平与临床治疗

The research of drug resistance gene in Old Silicosis Mycobacterium tuberculosis

目的分析老年矽肺结核病的耐药水平和7种耐药基因突变情况。方法 117例老年矽肺结核患者的临床分离株均做传统梯度药敏试验和聚合酶链反应-单链构象多态性(PCR-SSCP)分析。结果被测菌株传统药敏试验耐药率82.1%。7种耐药基因[链霉素(rpsL)、利福平(rpoB)、异烟肼(katG)、乙胺丁醇(embB)、吡嗪酰胺(pncA)、喹诺酮(gyrA)、卡那霉素(km)]突变率分别为78.1%、65.6%、52%、33.3%、37.5%、54.1%、28.1%。结论传统梯度药敏试验与7种耐药基因联合检测,证实耐药基因突变与耐药水平和治疗关系密切。

Objective To study the relationship between drug-resistance gene mutation and drug-resistance level in OLD Silicosis M.tuberculosis.Methods 117 M.tuberculosis strains isolated from clinical sputum specimens were analyzed by PCR-SSCP and traditional drug susceptibility tests.Results The gene mutation rate of SM（rpsL）,REP（rpoB）,INH（katG）,EMB（embB）,PZA（pncA）,Quinolones（gyrA） and KM（km） resistance of isolated strains were 78.1%,65.6%,52%,33.3%,37.5%,54.1% and 28.1%.Conclusion The gene mutation was is closely related to drug resistance level of M.tuberculosis.