牙髓拟杆菌的分离、培养、鉴定

Isolation and Cultural Identification Versus Direct Immunofluorescence Test of the Specimen Containing Bacteroides endodontalis

牙髓拟杆菌是产黑色素拟杆菌群中的一个新菌种。本试验通过对牙髓腔感染标本中分离、培养、鉴定各种细菌,发现牙髓拟杆菌主要分布在牙髓腔内,其存在与根尖周炎有无症状密切相关,此菌对氧敏感,用常规鉴定及气相色谱法与其它菌无法区别,而间接免疫荧光法是确定牙髓拟杆菌菌种和直接检测牙髓腔标本中有无此菌的一个快速、可靠的方法。

The laboratory examination methods chosen for 80 adult and 18 children with pulp chamber infection were as following: The specimens of dental root-canal were taken for isolation of bacterial colonies culture and identification of the anaerobic bacteria by means of various biochemical tests.agglutination reactions and detecting the metabolic products from PYG cultural fluids as well as by G+C content determination of DNA of bacteria and by means of using indirect immunofluorescence technique for detection of the target microbes.It was found that the detection rate of Black-Pigmented-Bacteroides(B.P.B.). was 77.5% in adult pulp chamber infection and 72.2% in similar infection in children. The majority of B.P.B. found were Bacteroides endodontalis and Bacteroides gingivalis.The results indicate that the detection rate of Bacteroides endodontalis appeared higher by the method of indirect immunofluorescence technique than by culture. The identification of Bacteroides endodontalis with the specimens from pulp chamber by the indirect immunofluorescence technique was found to be the most rapid and rather specific.