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HINT1基因在喉鳞状细胞癌中的表达和意义

Expression of gene in laryngeal squamous cell carcinoma and its clinical significance
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摘要 目的组氨酸三聚体核苷结合蛋白1(histidine triad nucleotide-binding protein1,HINT1)基因在喉鳞状细胞癌组织中的表达情况及其与患者临床病理特征的关系,探讨HINT1基因在喉鳞状细胞癌发病过程中的作用。方法通过实时定量PCR和免疫组织化学检测82例喉鳞状细胞癌组织和56例癌旁组织中HINT1mRNA和蛋白水平的表达,分析其蛋白表达与患者临床病理特征的关系。结果喉鳞状细胞癌组织HINT1mRNA水平显著低于癌旁组织(P<0.01),HINT1蛋白表达阳性率显著低于癌旁组织(P<0.05)。晚期喉鳞状细胞癌组织中HINT1蛋白表达显著低于早期喉鳞状细胞癌组织(P<0.05)。结论 HINT1在喉鳞状细胞癌中可能发挥抑癌基因的作用。 Objective To investigate the expression of histidine triad nucleotide-binding protein 1（） in laryngeal squamous cell carcinoma（LSCC） and its relationship with clinicopathological factors of the patients.Methods 82 cases of LSCC samples and 56 cases of pericancer samples were detected by real time PCR and immunohistochemical staining to analyze mRNA and protein levels,and the relationship between the expression of protein and clinicopathologic factors.Results The expression levels of mRNA in LSCC tissues were downregulated（ 0.01）,the positive rate of HINT1 protein expression was significantly lower in LSCC than in pericancer tissues（ 0.05）.HINT1 protein expression levels in LSCC tissues were associated with TNM stage of the patients（ 0.05）.Conclusion might play a role as a tumor suppressor gene in LSCC.
作者 王瑾 兰培丽 孙欣 金明洙
机构地区 中国医科大学附属盛京医院耳鼻喉科 中国医科大学附属盛京医院疼痛科
出处 《解剖科学进展》 CAS 2012年第3期251-254,共4页 Progress of Anatomical Sciences
关键词 HINT1 鳞状细胞癌 抑癌基因 免疫组化法 histidine triad nucleotide-binding protein 1 gene laryngeal squamous cell carcinoma tumor suppressor gene immunohistochemistry
分类号 R739.65 [医药卫生—肿瘤]
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参考文献12

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二级参考文献23

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