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Notch1信号抑制对成骨肉瘤细胞化疗药物敏感性的影响 被引量：3

Effects of Notch1 signaling inhibition in sensitivity of Osteosarcoma cells to chemotherapy
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摘要 目的研究抑制Notch1信号对成骨肉瘤细胞化疗药物敏感性的影响及部分机制。方法将人成骨肉瘤细胞系OS-732按γ-分泌酶抑制剂(GSI)作用与否分为GSI组和对照组;脂质体法转染真核细胞表达质粒;Realtime PCR检测NICD、Hes1mRNA表达;流式细胞仪(FACS)检测Annexin V-PE/7-AAD双标的细胞凋亡;MTT法检测化疗药物对细胞的抑制率。结果 GSI组NICD和Hes1 mRNA均较对照组明显降低(P<0.01)。GSI组化疗药物导致的瘤细胞抑制率和凋亡率均明显高于对照组。过表达Hes1可减弱GSI诱导的瘤细胞化疗药物敏感性增高。结论 GSI通过抑制Notch1受体激活和下游靶基因Hes1表达促进人成骨肉瘤细胞凋亡,从而增加人成骨肉瘤细胞对化疗药物的敏感性。 Objective To study the Effect of Notch1 signaling inhibition in sensitivity of osteosarcoma cells to chemotherapy.Methods The osteosarcoma cells OS-732 were divided into two groups with γ-secretase inhibitor（GSI） or without it.The plasmid was transfected by liposome.NICD and Hes1 mRNA was analyzed by Realtime PCR.The chemosensitivity and apoptosis rate of cells were measured by MTT and flow cytometry respectively.Results The level of NICD and Hes1 mRNA was reduced in group GSI,compared with that in control group（P0.01）.Meanwhile,the chemosensitivity and apoptosis rate of osteosarcoma cells in GSI group were higher than that in control gourp.However,the over-expression of Hes1 decreased the chemosensitivity of osteosarcoma cells which increased by GSI treatment.Conclusion GSI enhances the sensitivity of osteosarcoma cells to chemotherapy through promoting the cell apoptosis,which is induced by inhibition of Notch1 receptor activity and target gene Hes1 expression.
作者 高天君 杨达宇
机构地区 解放军第
出处 《局解手术学杂志》 2012年第3期285-287,共3页 Journal of Regional Anatomy and Operative Surgery
基金 国家重点实验室开放基金项目(SKLKF 201005)
关键词 人成骨肉瘤细胞 细胞凋亡 Γ-分泌酶抑制剂 NOTCH1 化疗敏感性 osteosarcoma cells cell apoptosis γ-secretase inhibitor Notch1 sensitivity to chemotherapy
分类号 R738.1 [医药卫生—肿瘤] R730.53 [医药卫生—肿瘤]
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参考文献11

  • 1Zhang P,Yang Y,Nolo R, et al. Regulation of NOTCH signaling by reciprocal inhibition of HES1 and Deltex 1 and its role in osteosarcoma invasiveness [J]. Oneogene,2010,29 (20) :2916-2926.
  • 2Tao J, Chen S, Lee B. Alteration of Notch signaling in skeletal development and disease[J]. Ann N Y Acad Sci ,2010,1192:257-268.
  • 3杨彩虹,陈安民,曾恒.紫杉醇与常规化疗药物对人骨肉瘤细胞的毒性比较[J].实用医学杂志,2008,24(18):3095-3098. 被引量：2
  • 4Shimojo H, Ohtsuka T, Kageyama R. Oscillations in notch signaling regulate maintenance of neural pmge[J]. Neuron ,2008,58 (1) :52-64.
  • 5施荣茂,连小华.毛囊干细胞巢研究现状[J].局解手术学杂志,2011,20(4):436-438. 被引量：2
  • 6Hozumi K, Negishi N,Tsuchiya I, et al. Notch signaling is necessary for GATA3 function in the initiation of T cell developm[J]. Eur J hnmunol,2008,38 (4) :977-985.
  • 7Robert Moreno A,Espinosa L, Sanchez MJ,et al. The notch pathway positively regulates programmed cell death during erythroid differentiation[J]. Leukemia,2007,21 (7) : 1496-1503.
  • 8Wang M,Wu L, Wang L, et al. Down-regulation of Notch l by gammasecretase inhibition contributes to cell growth inhibition and apoptosis in ovarian cancer cells A2780[J]. Biochem Biophys Res Commun,2010, 393 ( 1 ) : 144-149.
  • 9Perumalsamy LR, Nagala M, Satin A. Notch-activated signaling cascade interacts with mitochondrial remodeling proteins to regulate cell survival [J]. Proc Natl Acad Sci U S A,2010,107(15) :6882-6887.
  • 10Chadwick N,Zeef L,Portillo V, et al. Notch protection against apoptosis in T-ALL cells mediated by GIMAP5 [J]. Blood Cells Mol Dis, 2010,45 ( 3 ) : 201-209.

二级参考文献24

  • 1沈关心 周汝麟.现代免疫学实验技术[M] 2版[M].武汉：湖北科学技术出版社,2002.398-400.
  • 2Cotsarelis G,Sun TT,Lavker RM.Label-retaining cells reside in the bulge area of pilosebaceous unit:implications for follicular stem cells,hair cycle,and skin carcinogenesis[J].Cell,1990,61(7):1329-1337.
  • 3Blanpain C,Lowry WE,Geoghegan A,et al.Self-renewal,multipotency,and the existence of two cell populations within an epithelial stem cell niche[J].Cell,2004,118(5):635-648.
  • 4Nishimura EK,Suzuki M,Igras V,et al.Key roles for transforming growth factor beta in melanocyte stem cell maintenance[J].Cell Stem Cell,2010,6(2):130-140.
  • 5Inoue K,Aoi N,Sato T,et al.Differential expression of stem-cell-associated markers in human hair follicle epithelial cells[J].Lab Invest,2009,89(8):844-856.
  • 6Morris RJ,Liu Y,Marles L,et al.Capturing and profiling adult hair follicle stem cells[J].Nat Biotechnol,2004,22(4):411-417.
  • 7Mitsiadis TA,Barrandon O,Rochat A,et al.Stem cell niches in mammals[J].Exp Cell Res,2007,313(16):3377-3385.
  • 8Jaks V,Barker N,Kasper M,et al.Lgr5 marks cycling,yet long-lived,hair follicle stem cells[J].Nat Genet,2008,40(11):1291-1299.
  • 9Snippert HJ,Haegebarth A,Kasper M,et al.Lgr6 marks stem cells in the hair follicle that generate all cell lineages of the skin[J].Science,2010,327(5971):1385-1389.
  • 10Zhang YV,Cheong J,Ciapurin N,et al.Distinct self-renewal and differentiation phases in the niche of infrequently dividing hair follicle stem cells[J].Cell Stem Cell,2009,5(3):267-278.

同被引文献69

  • 1郑毅,董馨,陆江阳,赵敏.骨关节炎患者软骨及滑膜高迁移率族蛋白1的表达[J].中华老年医学杂志,2006,25(3):173-176. 被引量：1
  • 2Goodwin GH, Johns EW. Isolation and characterisation of two calf-thy- mus chromatin non-histone proteins with high contents of acidic and basic amino acids[ J]. Eur J Biochem, 1973,40( 1 ) :215 - 219.
  • 3Wang H,Bloom O,Zhang M, et al. HMG-I as a late mediator of endo- toxin lethality in mice[ J]. Science, 1999,285:248 -251.
  • 4Yang H, Antoine DJ, Andersson U, et al. The many faces of HMGB1 : molecular structure-functional activity in inflammation, apoptosis, and chemotaxis[ J]. J Leukoc Bio1,2013,93 (6) :865 - 873.
  • 5Lamore SD, Cabello CM,Wondrak GT. HMGBl-directed drug discovery targeting cutaneous inflammatory dysregulation [ J ]. Curt Drug Metab, 2010,11 (3) :250 - 265.
  • 6Tang D, Kang R, Zeh H J, et al. High-mobility group box 1, oxidative stress, and disease [ J ]. Antioxid Redox Signal, 2011,14 ( 7 ) : 1315 - 1335.
  • 7Zhu S, Li W, Ward MF, et al. High mobility group box 1 protein as a po- tential drug target for infection-and injury-elicited inflammation[ J. In-flamm Allergy Drug Targets,2010,9( 1 ) :60 -72.
  • 8Bell cw, Jiang W, Reich CF 3rd, et al. The extracellular release of HMGB1 during apoptotic cell death [ J ]. Am J Physiol Cell Physiol, 2006,291 (6) :C1318 - 1325.
  • 9Sims GP, Rowe DC, Rietdijk ST, et al. HMGB1 and RAGE in inflamma- tion and cancer[J]. Annu Rev Immunol,2010,28:367 -388.
  • 10Xu D, Young J, Song D, et al. Heparan sulfate is essential for high mob- ility group protein 1 ( HMGB1 ) signaling by thereceptor for advanced glycationend products ( RAGE ) [ J ]. J Biol Chem, 2011,286 ( 48 ) : 41736 -41744.

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局解手术学杂志
2012年 第3期

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