2-CAdo对脑缺血再灌注后血清及皮质TNF-α含量的影响

Influence of 2-CAda on TNF-α levels in serum and cortex after focal cerebral ischemia-reperfusion

目的:研究大鼠局灶性脑缺血再灌注后非选择性腺苷受体激动剂2-CAdo(2-Chloroadenosine)对血清及皮质肿瘤坏死因子-α(TNF-α)含量的影响,从而进一步探讨腺苷受体激动剂的神经保护作用。方法 采用线栓法制备大鼠局灶性脑缺血再灌注模型,于缺血后即刻经静脉注射2-CAdo,应用放射免疫法测定缺血1h再灌注6h后血清及皮质TNF-α的含量。结果 2-CAdo能显著降低脑缺血再灌注后血清及皮质TNF-α的含量。结论 2-CAdo可通过降低脑缺血再灌注后增高的TNF-α水平,达到神经保护作用。

Objective To study the effect of nonselective adenosine receptor agonist 2-chloroadenosine(2-CAdo ) on trmor necrosis factor-alpha (TNF-α) levels in serum and cortex of rats after focal cerebral ischemia-reperfusion. So as to explore the neuroprotective mechanism of adensine receptor agonist. Methods We established the focal cerebral ischemia-reperfusion model with thread embolish of middle cerebral artery, injected 2-CAdo via vein as soon as ischemia, and determined the levels of TNF-a in serum and cortex after 1 hour ischemia and 6 hour reperfusion by RIA. Results 2-CAdo can decrease the levels of TNF-α in serum and cortex after focal cerebral ischemia-reperfusion. Conclusion 2-CAdo showed a potent neuroprotective effect through decreasing the increased TNF-a after cerebral ischemia-reperfusion.