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bFGF对跟腱成纤维细胞创伤愈合影响的体外研究 被引量:4

Effect of Basic Fibroblast Growth Factoron Achilles Tendon Fibroblasts Wound Healingin Vitro
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摘要 目的 :研究bFGF在跟腱损伤修复中的作用及机制。方法 :采用体外创伤愈合和细胞运动模型及MTT方法 ,分别对bFGF作用下 ,跟腱成纤维细胞创伤愈合、运动与增殖行为进行分析。结果 :bFGF浓度为 5ng/ml时 ,即可显著地促进跟腱成纤维细胞的创伤愈合与增殖 ,当bFGF浓度提高至 10 0ng/ml,这种促进作用达到最大值。而bFGF对跟腱成纤维细胞的运动行为并无显著影响。结论 Objective :Tostudytheroleofbasicfibroblastgrowthfactor(bFGF)onAchillestendonhealing .Method :Ef fectsofbFGFonwoundhealing ,migrationandproliferationofculturedAchillestendonfibroblastswerestudiedinwoundclosure andmigrationmodelsaswellasbyMTTmethodrespectively .Results :bFGFsignificantlyenhancedwoundhealingandprolifera tionofAchillestendonfibroblastsattheconcentrationof 5ng/ml ,andthisenhancementwasmaximuminthepresenceof 10 0ng/mlbFGF .However ,bFGFhadnoeffectonthemigrationofAchillestendonfirbroblasts .Conclusion :Theenhancementof woundhealingbybFGFmaybecausedbycellproliferation .
出处 《中国矫形外科杂志》 CAS CSCD 2000年第5期456-458,共3页 Orthopedic Journal of China
关键词 跟腱创伤 体外 愈合 成纤维细胞增殖 BFGF Achillestendon Invitro Healing Proliferation bFGF
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