带血运的组织移植对损伤脊髓细胞凋亡的影响

Effect of the vascularized tissue transplants on apoptosis after spinal cord injury

目的 :探讨带蒂的大网膜和椎旁肌移植覆盖脊髓对脊髓损伤后细胞凋亡的影响。方法 :应用改良的 Allen's法致伤脊髓 ,将动物分为单纯脊髓损伤组 (A组 )、损伤 +椎旁肌组 (B组 )、损伤+大网膜组 (C组 ) .移植后 1、3、7、14、2 8天对脊髓损伤区进行细胞凋亡的检测 (TU NEL)以及 Bcl- 2蛋白表达的测定 (免疫组化法 )。结果 :A、B、C组中均发现凋亡细胞及 BCL- 2蛋白阳性表达 ,图象分析发现 ,细胞凋亡率为 A>B>C,Bcl- 2蛋白阳性表达顺序为 C>B>A。结论

Objectives:To investigate the effect of the vascularized tissue transplants on apoptosis after spinal cord injury (SCI).Methods:The adult experimental rats received lumbar spinal cord contusion, and were divided into three groups:The SCI group(group A),contusion with pedicle paraspinal muscle transferred group(group B), contusion with the pedicle omentum transferred group(group C). The apoptosis of spinal slice from the injured spinal cord were examined by the methods of the terminal deoxynucleotidal transferase mediated DUTP biotin nick end labelling (TUNEL) reaction and the expression of Bcl 2 was measured immunohistochemically in 1,3,7,14 and 28 days post operation respectively.Results:The rate of apoptosis sequence and the strong expression sequence of Bcl 2 protein expression were found in all three groups. The former was A>B>C ( P <0.005), and the later was C>B>A ( P <0.05). These sequences were parallel by a significant improvement in neurological function recovery. Conclusions:It indicates that the pedicle omentum transplants can inhibit the apoptosis and improve rats hind limb function after SCI.