摘要
了解海洛因依赖者输血传播 (TT)病毒感染状况 ;研究其致病性及病毒特征 ;探讨感染传播途径。方法··:采用巢式PCR和ELISA法检测患者血清TT病毒DNA和抗体IgG。结果·· :286例血清标本TT病毒DNA和抗体IgG的阳性率分别为7.0%和9.4 % ;无一例标本同时检出DNA和抗体IgG ;ALT升高和正常组TT病毒感染阳性率无显著性差异 (P>0.05〕。结论··:海洛因依赖者中存在TT病毒感染和无症状携带状态,感染不依赖其它肝炎病毒而单独存在 ;TT病毒可能是一种新的自限性DNA病毒 ,其对肝脏的损害作用不确切 ;静脉注射不是海洛因依赖者TT病毒感染的主要途径。
Objective:Todetecttheinfectiverates,studythepathogeneticroleandviralcharacter istics,andsearchforrouteoftransmissionoftransmitted -transfusion(TT)virusinheroinaddicts. Method:Nested - polymerasechainreaction(nPCR)andenzyme -linkedimmunosorbentassay(ELISA) wereperformedtodetectTTvirusDNAandanti -TTvirusIgGinserumsamplesfrom patients. Result:ThepositiveratesofTTvirusDNAandanti -TTvirusIgGwere7.0 %and9.4 %respectively among286samples.NopositiveresultofbothTTvirusDNAandanti -TTvirusIgGwasdetectedin thesamesample.ThepositiverateofTTvirusDNAinelevatedalanineaminotransferase(ALT)level groupwasnotsignificantlydifferentfromthatinthenormalALTgroup(χ2=0.054,P>0.05).Con clusion:ThereexistsTTvirusinfectionandasymptomaticcarriage,whichareindependentonhepatitis viruses,amongheroinaddicts.TTvirusisprobablyanewself -limitedDNAvirus,buttherewasno obviousbiochemicalorhistologicalevidenceforthedamageofliver.TTvirusmaynotbetransmitted mainlybyintravenousinjectioninheroinaddicts.
出处
《中国药物依赖性杂志》
CAS
CSCD
2000年第2期118-120,共3页
Chinese Journal of Drug Dependence
关键词
输血传播病毒
海洛因依赖
PCR
ELISA
TTvirus
heroinaddict
polymerase chainreaction
enzyme-linked immunosorbent assay
