线虫中RAB-27与其效应物RBF-1调控致密核心囊泡的栓系和锚定过程

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摘要致密核心囊泡的转运、栓系、锚定和成熟过程的分子机制尚不清楚.本文以秀丽隐杆线虫(简称线虫)为模式生物来研究致密核心囊泡分泌过程中Rab蛋白及其效应物的功能.实验认为,RAB-27/AEX-6,而非RAB-3,是神经肽释放所必需的.通过全内反射荧光显微镜技术分析了质膜附近致密核心囊泡的运动情况,阐述了RAB-27/AEX-6和囊泡的栓系相关,而RAB-27的效应物Rabphilin/RBF-1则是早期囊泡和膜栓系及稳定锚定所必需的.

作者冯婉娟梁滔于俊伟周围章永登吴政星徐涛

机构地区华中科技大学生命科学与技术学院中国科学院生物物理研究所

出处《中国科学：生命科学》 CSCD 北大核心 2012年第5期365-373,共9页 Scientia Sinica(Vitae)

基金国家重点基础研究发展计划(批准号:30870564,90913022) 国家自然科学基金(批准号:2010CB833701)资助项目

关键词分泌致密核心囊泡 RAB-3 RAB-27 TIRFM

分类号 Q51 [生物学—生物化学]

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线虫中RAB-27与其效应物RBF-1调控致密核心囊泡的栓系和锚定过程

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