依那普利对肾性高血压大鼠RAS系统及NO影响的实验研究

The Effect of Enalapril on RAS System and NO Level in Renal \;Hypertension Rat

目的 :了解血压增高、肾素 (PRA)、血管紧张素 (Ang )分泌增强时对 ET- 1、NOS及 NO生成的影响方法 :制造肾性高血压大鼠模型 ,并灌喂依那普利液体 ,检测血或组织 Ang 、ET- 1、NOS及 NO等水平。结果 :肾动脉狭窄使 PRA、Ang 、ET- 1生成增高 ,NOS活性及 NO生成下降 ,血压升高与 Ang 及 PRA水平有明显正相关 ,与 NO呈负相关 ,8周后血 Ang 水平逐渐恒定或减低 ,而组织匀浆 Ang 升高 ,依那普利可改善上述状况。推论 :ACEI作用部位不仅存在于循环系统中 ,也存在于不同的组织中 ,组织型肾素 -血管紧张素系统作为

Objective:To study the effect of enalapril on high blood pressure, PRA and AngⅡ for ET 1 ,NOS and NO productions in the renal hypertension rat. Methods:Left renal artery was partially obstructed by ligation with the residual diameter of 0.22 mm. The levels of AngⅡ,ET 1,NOS and NO in blood or tissue samples acquired from renal hypertension rat were measured by immuno radioactive assay. Results:PRA,AngⅡ and ET 1 releases were increased and NOS activity or NO level decreased in rats with renal artery stenosis. There were a positive relationship between blood pressure and plasma AngⅡ or PRA level,and a negative relationship with NO productions. The AngⅡ releases in gradually slowed down and examined stable or even decrease while the tissue AngⅡ was increased markedly 8 weeks after the operation. The ACEI enalapril attenuated the increase of tissue AngⅡ,PRA and ET 1 in renal hypertensive rats,and enhenced the recovery of NO level. The incressed tissue renin angiotesin activity seems to participate the mechanism of persistent hypertension during late stage. Conclusion:ACEI play an important role in many systems,especially in tissue renin angiotensin system.