高钠盐摄入对钠泵抑制因子水平和钠-钾泵活性的影响

Effects of Chronic High Salt Diet Intake on Sodium-Pump InhibitorLevels and Na_)+-K_+-Pump Activity in Rats

目的 :探讨长期摄入高钠盐饮食引发高血压的机理。方法 :以普通饲料和 1.5 % Na Cl溶液饲喂雄性 Sprague-Dawley大鼠 6周 ,颈动脉插管直接测量血压 ,EL ISA检测血浆中、肾上腺和下丘脑组织中哇巴因样物质 (oubain- likecom pound,OL C)和前海葱苷原 A样物质 (proscillaridin- likecom pound,PL C)免疫反应性 ,在有和无哇巴因存在的条件下水解 ATP测定 Na+ - K+ - ATP酶活性。结果 :高血压组动脉血压较对照组明显升高 (P<0 .0 0 1) ,体重、肾重、肾重 /体重比率、血浆中及肾上腺和下丘脑组织中OL C和 PL C水平分别明显高于对照组 (P<0 .0 0 1) ,肾脏Na+ - K+ - ATP酶活性明显低于对照组 (P<0 .0 0 1)。结论 :长期摄入高钠盐饮食可能通过体液容量扩张刺激肾上腺和下丘脑分泌 OL C和 PL C入血循环 ,抑制组织细胞特别是血管平滑肌细胞膜 Na+ - K+ - ATP酶活性 ,引起细胞内 Na+和游离 Ca2 +浓度升高而导致血压升高。

Aim: To study the pathogenesis of hypertension induced by chronic high salt diet intake. Methods:Male Sprague Dawley rats weighing approximately 200 g were divided into two groups. The control group received normal diet and deionized water and the high salt group received normal diet and 1.5% NaCl solution for 6 weeks, before study, direct blood pressure was recorded by a carotid artery catheter. Oubain like compound (OLC) and proscillaridin like compound (PLC) immunoreactivities in plasma, adrenal and hypothalamus were measured. Hydrolysis of ATP in the absence or presence of oubain was used to assay kidney microsomes Na + K + ATPase activity. Results:The high salt diet increased arterial pressure and OLC and PLC immunoreactivities in plasma, adrenal and hypothalamus, and decreased kidney microsomes Na + K + ATPase activity compared with control group. Conclusion:The present data indicate that chronic high salt diet intake may induce hypertension through stimulation of release OLC and PLC from adrenal and/or hypothalamus into circulation, which may inhibite Na + K + ATPase activity.