Lumican蛋白在胰腺导管腺癌间质中的表达及其与Ki-67、VEGF及突变型P53表达的相关性

Lumican protein expression in pancreatic ductal adenocarcinoma:clinical significance and correlation with expression of Ki-67,VEGF and mutated P53

目的:观察细胞外基质蛋白Lumican在胰腺导管腺癌(pancreatic ductal adenocarcinoma,PDA)中表达特征,分析Lumican与Ki-67、VEGF、突变型P53等肿瘤恶性表型相关分子的关联.方法:采用免疫组织化学染色(IHC)和逆转录-聚合酶链式反应(RT-PCR)检测PDA原发灶及对应癌旁胰腺组织中Lumican表达.IHC检测PDA原发灶Ki-67、VEGF及突变型P53表达.用SPSS软件行统计学分析.结果:PDA原发灶中,Lumican表达在mRNA及蛋白质水平均明显高于癌旁胰腺组织.就该蛋白在癌灶中的分布特性而言,Lumican蛋白主要定位于癌间质,阳性表达率为83.0%(83/100).低分化PDA中,癌间质过表达Lumican与TNM分期相关(x2=6.446,P<0.05),与年龄、性别、淋巴结转移、远处转移等无明显相关.高中分化PDA中,癌间质过表达Lumican与临床病理特征无关,而与Ki-67(r=-0.28,P=0.017)、VEGF(r=-0.264,P=0.025)及突变型P53(r=-0.253,P=0.032)表达呈明显负相关.结论:Lumican在PDA原发灶中表达高于癌旁胰腺组织,主要分布于癌间质.Lumican在癌间质过表达与低分化PDA的TNM分期相关,与高、中分化PDA的Ki-67、VEGF及突变型P53表达呈负相关.

AIM：To detect the expression of Lumican in pancreatic ductal adenocarcinoma and to analyze its correlation with expression of Ki-67,VEGF and mutated P53.METHODS：One hundred surgically resected cancer samples collected from patients with pathologically confirmed pancreatic ductal adenocarcinoma and 15 tumor-adjacent pancreatic tissue samples were used in the study.Lumican expression in these tissue samples was detected by immunohistochemistry（IHC）and reverse transcription-polymerase chain reaction（RTPCR）.Expression of Ki-67,VEGF and mutated P53 was detected by IHC.RESULTS：The expression levels of Lumican mRNA and protein in cancer tissue were significantly higher than those in tumor-adjacent tissue.The positive rate of Lumican expression in tumor stroma was 83.0%（83/100）.In poorly differentiated samples,stromal expression of Lumican was significantly correlated with TNM stage（？2=6.446,P0.05）,but not with age,gender,lymph node metastasis or distant metastasis.In well differentiated samples,stromal expression of Lumican had a significant negative correlation with expression of Ki-67（r=-0.28,P=0.017）,VEGF（r=-0.264,P=0.025）and mutated P53（r =-0.253,P=0.032）,but had no correlation with pathological characteristics of pancreatic ductal adenocarcinoma.CONCLUSION：Lumican expression is higher in pancreatic ductal adenocarcinoma than in tumor-adjacent tissue and correlates with TNM stage in poor differentiated samples.There is a negative correlation between expression of Lumican and that of Ki-67,VEGF and mutated P53 mutation in well differentiated samples.