通用引物巢式PCR法对哈萨克族食管鳞癌HPV感染的检测

Detection of human papilloma virus in esophageal cancer in Xinjiang Kazakh patients by general primer-mediated polymerase chain reaction

目的:应用通用引物巢式PCR技术检测新疆哈萨克族食管鳞癌人乳头瘤病毒(HPV)的感染率,并探讨HPV与新疆哈萨克族食管鳞癌的关系.方法:提取新疆哈萨克族食管鳞癌及正常食管组织的DNA,应用通用引物HPV MY09/11及HPV G5+/6+进行巢式PCR,检测新疆哈萨克族食管鳞癌中HPV的感染率.结果:巢式PCR法扩增后检测到食管鳞癌中HPV感染率为66.67%,正常对照组为12.12%,两者差异有统计学意义(P<0.05).结论:本实验结果表明HPV可能是新疆哈萨克族食管癌发生的一个重要病因学因素,结合通用引物巢式PCR法可以更加方便可靠的检测HPV-DNA.

AIM： To detect human papillomavirus （HPV） in esophageal squamous cell carcinoma （ESCC） in Xinjiang Kazakh patients by universal primermediated nested PCR to investigate the relationship between HPV infection and ESCC. METHODS： DNA was isolated from ESCC and healthy esophageal mucosal specimens from Xinjiang Kazakh adults and used to amplify HPV using universal primers HPV MY09/11 and HPVspecific primers HPV G5＋/6＋ by nested PCR. The rate of HPV infection was then calculated. RESULTS： The rate of HPV infection was 66.67% in the ESCC group and 12.12% in the healthy control group.