触珠蛋白在急性心肌梗死患者血清中的表达

Expression of haptoglobin in sera of patients with acute myocardial infarction

目的探讨触珠蛋白(Haptoglobin,Hp)在急性心肌梗死(Acute myocardial infarction,AMI)患者血清中的表达情况,为该疾病的诊断与治疗提供新思路。方法收集30例确诊的AMI患者和30例体检证实为健康人群组的血清,去除血清高丰度蛋白后,采用双向电泳(2-DE)分离血清蛋白,获得的电泳图谱经ImageMaster5.00软件分析,差异明显的蛋白点经胶内酶解后,行基质辅助激光解吸电离飞行时间质谱(Matrix-assisted laser desorption-ionization-time of flight-mass spectrometry,MALDI-TOF-MS)分析,检测Hp的表达。结果 AMI患者与健康人群组血清的电泳图谱相似;MALDI-TOF-MS分析显示,AMI患者血清中Hp表达量明显高于健康人群组(P<0.05)。结论 Hp在AMI患者血清中表达量升高,其有望成为AMI患者的特异性诊断标记物。

Objective To investigate the expression of haptoglobin（Hp） in sera of patients with acute myocardial infarction（AMI） and provide a novel route for diagnosis and treatment of the disease.Methods Serum samples were collected from 30 patients with confirmed AMI and 30 healthy persons confirmed by physical examination,in which high abundance proteins were removed,and remained proteins were separated by two-dimensional electrophoresis（2-DE） and the obtained electrophoretic profile was analyzed by using ImageMaster5.00 software.Significantly differential protein spots were digested with enzyme in the gel,and determined for expression of Hp by matrix-assisted laser desorption-ionization-time of flight-mass spectrometry（MALDI-TOF-MS）.Results The 2-DE profiles of patients with AMI were similar to those of healthy persons.The expression level of Hp in sera of patients with AMI was significantly higher than that in healthy persons（P 0.05）.Conclusion The expression level of Hp increased in sera of patients with AMI,indicating that Hp might be used as a specific marker for diagnosis of AMI.